PMID- 19671014
OWN - NLM
STAT- MEDLINE
DCOM- 20091006
LR  - 20090812
IS  - 0031-949X (Print)
IS  - 0031-949X (Linking)
VI  - 99
IP  - 9
DP  - 2009 Sep
TI  - Specific genes from the potato brown rot strains of Ralstonia solanacearum and 
      their potential use for strain detection.
PG  - 1105-12
LID - 10.1094/PHYTO-99-9-1105 [doi]
AB  - Ralstonia solanacearum is the agent of bacterial wilt infecting >200 different 
      plant species covering >50 botanical families. The genus R. solanacearum can be 
      classified into four phylotypes and each phylotype can be further subdivided into 
      sequevars. The potato brown rot strains of R. solanacearum from phylotype IIB, 
      sequevar 1 (IIB1), historically known as race 3, biovar 2 strains, are 
      responsible for important economic losses to the potato industry and threaten 
      ornamental crop production worldwide. Sensitive and specific detection methods 
      are required to control this pathogen. This article provides a list of 70 genes 
      and 15 intergenes specific to the potato brown rot strains of R. solanacearum 
      from phylotype IIB1. This list was identified by comparative genomic 
      hybridization on microarray and subsequent polymerase chain reaction validation 
      with 14 IIB1 strains against 45 non-IIB1 strains that covered the known genetic 
      diversity in R. solanacearum. The microarray used consisted of the previously 
      described microarray representative of the phylotype I strain GMI1000, to which 
      were added 660 70-mer oligonucleotides representative of new genomic islands 
      detected in the phylotype IIB1 strain IPO1609. The brown rot strain-specific 
      genes thus identified were organized in nine clusters covering 2 to 29 genes 
      within the IPO1609 genome and 6 genes isolated along the genome. Of these 
      specific genes, 29 were parts of mobile genetic elements. Considering the known 
      instability of the R. solanacearum genome, we believe that multiple probes are 
      required to consistently detect all IIB1 strains and we recommend the use of 
      probes which are not part of genetic mobile elements.
FAU - Guidot, A
AU  - Guidot A
AD  - CIRAD, UMR PVBMT, Saint Pierre, La Reunion, F-97410, France.
FAU - Elbaz, M
AU  - Elbaz M
FAU - Carrere, Sebastien
AU  - Carrere S
FAU - Siri, M I
AU  - Siri MI
FAU - Pianzzola, M J
AU  - Pianzzola MJ
FAU - Prior, P
AU  - Prior P
FAU - Boucher, C
AU  - Boucher C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Phytopathology
JT  - Phytopathology
JID - 9427222
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - *Genes, Bacterial
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
MH  - Ralstonia solanacearum/*genetics
EDAT- 2009/08/13 09:00
MHDA- 2009/10/07 06:00
CRDT- 2009/08/13 09:00
PHST- 2009/08/13 09:00 [entrez]
PHST- 2009/08/13 09:00 [pubmed]
PHST- 2009/10/07 06:00 [medline]
AID - 10.1094/PHYTO-99-9-1105 [doi]
PST - ppublish
SO  - Phytopathology. 2009 Sep;99(9):1105-12. doi: 10.1094/PHYTO-99-9-1105.