PMID- 19671249
OWN - NLM
STAT- MEDLINE
DCOM- 20090922
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 33
IP  - 5
DP  - 2009 Jun
TI  - A comparison between experimental and authentic blood/serum ratios of 
      3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyamphetamine.
PG  - 283-6
AB  - This paper compares the blood-to-serum distribution (B/S ratio) of 
      3,4-methylenedioxymethamphetamine (MDMA) and its major metabolite 
      3,4-methylenedioxyamphetamine (MDA). B/S ratios were determined by liquid 
      chromatography-tandem mass spectrometry analysis following liquid-liquid 
      extraction as a function of the hematocrit value (experimental specimens) and in 
      blood and corresponding serum samples (n = 63) from 16 healthy volunteers 
      participating in a controlled driving experiment (authentic specimens). A 
      regression analysis to calculate the B/S ratio was performed followed by an 
      analysis of covariances (ANCOVA). A linear relationship between the hematocrit 
      value and the B/S ratio of both MDMA and MDA could be established from the 
      experimental data. For MDMA, the regressions provided mean B/S ratios of 1.22 and 
      1.26 for the experimental setting and the authentic samples, respectively. For 
      MDA, the analysis determined slopes of 1.15 and 1.27 for the experimental setting 
      and field study, respectively. ANCOVA revealed that the method of determination 
      (experimental vs. authentic specimens) did not influence the resulting slopes. A 
      conversion factor of 0.80 may give an adequate estimate to derive the serum 
      concentration for MDMA if only the concentration in whole blood is known, whereas 
      such a definitive factor could not be established for MDA because of its very low 
      levels in authentic samples.
FAU - Boy, Regine Garcia
AU  - Boy RG
AD  - Institute of Legal Medicine and Forensic Medicine, University Hospital, Voss-Str. 
      2, 69115 Heidelberg, Germany. regine.garcia@med.uni-heidelberg.de
FAU - Henseler, Joerg
AU  - Henseler J
FAU - Ramaekers, Johannes G
AU  - Ramaekers JG
FAU - Mattern, Rainer
AU  - Mattern R
FAU - Skopp, Gisela
AU  - Skopp G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Hallucinogens)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*blood
MH  - Atmospheric Pressure
MH  - Chromatography, High Pressure Liquid
MH  - Hallucinogens/*blood
MH  - Hematocrit
MH  - Humans
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*blood
MH  - Regression Analysis
MH  - Substance Abuse Detection/*methods
MH  - Tandem Mass Spectrometry/methods
EDAT- 2009/08/13 09:00
MHDA- 2009/09/23 06:00
CRDT- 2009/08/13 09:00
PHST- 2009/08/13 09:00 [entrez]
PHST- 2009/08/13 09:00 [pubmed]
PHST- 2009/09/23 06:00 [medline]
AID - 10.1093/jat/33.5.283 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2009 Jun;33(5):283-6. doi: 10.1093/jat/33.5.283.