PMID- 19671414
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20090812
IS  - 1672-1977 (Print)
IS  - 1672-1977 (Linking)
VI  - 7
IP  - 8
DP  - 2009 Aug
TI  - [Protective effects of astilbin on renal ischemia-reperfusion injury in rats].
PG  - 753-7
LID - 10.3736/jcim20090809 [doi]
AB  - OBJECTIVE: To investigate the protective effects of astilbin on renal 
      ischemia-reperfusion (IR) injury in rats. METHODS: Twenty-four male SD rats, two 
      months old, were randomly allocated into three groups: sham-operated group (n=8), 
      untreated group (n=8) and astilbin group (n=8). Rats in the untreated group and 
      the astilbin group underwent temporary renal artery occlusion to induce IR 
      injury. The rats in the astilbin group were intraperitoneally injected with 12 
      mg/mL astilbin at a dose of 30 mg/kg from 3 day before IR injury until to be 
      sacrificed once per day, and rats in the untreated group were injected with equal 
      volume of normal saline at the same time. After 6-hour reperfusion, blood urea 
      nitrogen (BUN) and serum creatinine (SCr) and histological changes of the renal 
      tissues were detected to evaluate renal injury. Expressions of monocyte 
      chemoattractant protein-1 (MCP-1) mRNA and protein in the renal tissues and the 
      serum contents of interleukin-6 (IL-6) and IL-1beta were also measured with 
      semi-quantitative reverse transcription-polymerase chain reaction, Western 
      blotting or enzyme-linked immunosorbent assay. RESULTS: Compared with the 
      untreated group, BUN and SCr levels were significantly decreased in the astilbin 
      group after 6-hour reperfusion (P<0.01), and similar results were also found in 
      histological examination. The expressions of MCP-1 mRNA and protein in renal 
      tissues in the astilbin group were lower than those in the untreated group. The 
      serum contents of IL-6 and IL-1beta were decreased in the astilbin group as 
      compared with the untreated group (P<0.01). CONCLUSION: Astilbin can ameliorate 
      kidney IR injury in rats by inhibiting the production of chemokine MCP-1 and 
      cytokines IL-6 and IL-1beta.
FAU - Song, Shao-Hua
AU  - Song SH
AD  - Organ Transplantation Center, Changzheng Hospital, Second Military Medical 
      University, Shanghai 200003, China.
FAU - Shen, Xiao-Yun
AU  - Shen XY
FAU - Liu, Fang
AU  - Liu F
FAU - Tang, Yi
AU  - Tang Y
FAU - Wang, Zhen-Meng
AU  - Wang ZM
FAU - Fu, Zhi-Ren
AU  - Fu ZR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Xi Yi Jie He Xue Bao
JT  - Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
JID - 101199657
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonols)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Messenger)
RN  - 29838-67-3 (astilbin)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/metabolism
MH  - Drugs, Chinese Herbal/pharmacology
MH  - Flavonols/*pharmacology
MH  - Interleukin-1beta/blood
MH  - Interleukin-6/blood
MH  - Kidney/*blood supply
MH  - Male
MH  - Protective Agents/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*drug therapy
EDAT- 2009/08/13 09:00
MHDA- 2011/02/04 06:00
CRDT- 2009/08/13 09:00
PHST- 2009/08/13 09:00 [entrez]
PHST- 2009/08/13 09:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
AID - jcim20090809 [pii]
AID - 10.3736/jcim20090809 [doi]
PST - ppublish
SO  - Zhong Xi Yi Jie He Xue Bao. 2009 Aug;7(8):753-7. doi: 10.3736/jcim20090809.