PMID- 19671672
OWN - NLM
STAT- MEDLINE
DCOM- 20100106
LR  - 20181201
IS  - 1557-3125 (Electronic)
IS  - 1541-7786 (Linking)
VI  - 7
IP  - 8
DP  - 2009 Aug
TI  - Differential influence of normal and cancer-associated fibroblasts on the growth 
      of human epithelial cells in an in vitro cocultivation model of prostate cancer.
PG  - 1212-23
LID - 10.1158/1541-7786.MCR-09-0073 [doi]
AB  - The prostate is composed of a number of different cell populations. The 
      interaction between them is crucial for the development and proper function of 
      the prostate. However, the effect of the molecular cross talk between these cells 
      in the course of carcinogenesis is still unclear. Employing an approach wherein 
      immortalized epithelial cells and immortalized human fibroblasts were cocultured, 
      we show that normal associated fibroblasts (NAF) and cancer-associated 
      fibroblasts (CAF) differentially influenced the growth and proliferation of 
      immortalized human prostate epithelial cells. Whereas NAFs inhibited the growth 
      of immortalized epithelial cells but promoted the growth of metastatic PC-3 
      cells, CAFs promoted the growth of immortalized epithelial cells but not of PC-3. 
      Cytokine arrays revealed that NAFs secreted higher levels of tumor necrosis 
      factor-alpha compared with CAFs whereas CAFs secreted higher levels of 
      interleukin-6 (IL-6) compared with NAFs. The growth-inhibiting effects of NAFs 
      were counteracted by the addition of IL-6, and the growth-promoting effects 
      exerted by the CAFs were counteracted by tumor necrosis factor-alpha. 
      Furthermore, CAFs induced the migration of endothelial cells in an IL-6-dependent 
      manner. Here, we show that normal fibroblast cells have a protective function at 
      very early stages of carcinogenesis by preventing immortalized epithelial cells 
      from proliferating and forming new blood vessels whereas CAFs aid immortalized 
      epithelial cells to further develop.
FAU - Paland, Nicole
AU  - Paland N
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Herzl, 1, 
      Rehovot 76303, Israel.
FAU - Kamer, Iris
AU  - Kamer I
FAU - Kogan-Sakin, Ira
AU  - Kogan-Sakin I
FAU - Madar, Shalom
AU  - Madar S
FAU - Goldfinger, Naomi
AU  - Goldfinger N
FAU - Rotter, Varda
AU  - Rotter V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090811
PL  - United States
TA  - Mol Cancer Res
JT  - Molecular cancer research : MCR
JID - 101150042
RN  - 0 (Androgens)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Androgens/pharmacology
MH  - Cell Line, Transformed
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/pharmacology
MH  - Epithelial Cells/*cytology/drug effects
MH  - Fibroblasts/drug effects/metabolism/*pathology
MH  - Humans
MH  - Interleukin-6/metabolism/pharmacology
MH  - Male
MH  - *Models, Biological
MH  - Prostatic Neoplasms/*pathology
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
EDAT- 2009/08/13 09:00
MHDA- 2010/01/07 06:00
CRDT- 2009/08/13 09:00
PHST- 2009/08/13 09:00 [entrez]
PHST- 2009/08/13 09:00 [pubmed]
PHST- 2010/01/07 06:00 [medline]
AID - 1541-7786.MCR-09-0073 [pii]
AID - 10.1158/1541-7786.MCR-09-0073 [doi]
PST - ppublish
SO  - Mol Cancer Res. 2009 Aug;7(8):1212-23. doi: 10.1158/1541-7786.MCR-09-0073. Epub 
      2009 Aug 11.