PMID- 19673035
OWN - NLM
STAT- MEDLINE
DCOM- 20091208
LR  - 20211203
IS  - 1332-8166 (Electronic)
IS  - 0353-9504 (Print)
IS  - 0353-9504 (Linking)
VI  - 50
IP  - 4
DP  - 2009 Aug
TI  - Human umbilical cord blood-derived stromal cells suppress xenogeneic immune cell 
      response in vitro.
PG  - 351-60
AB  - AIM: To explore immunological properties of human umbilical cord blood-derived 
      stromal cells (hUCBDSC) and their effect on xenogeneic immune cells in vitro. 
      METHODS: Immunological phenotype of freshly isolated and cryopreserved hUCBDSCs 
      was evaluated by flow cytometry. Xenogeneic splenic T-cells were stimulated by 
      phytohemaglutinin A (PHA) or dendritic cells in the absence or presence of 
      hUCBDSCs. T-cell proliferation was measured by cell counting kit-8 after 7-day 
      incubation. The proportion of apoptotic cells and CD4+CD25+Foxp3+ regulatory 
      T-cells (Tregs) was determined in T-cells activated by PHA in the absence or 
      presence of hUCBDSCs by flow cytometry. Phenotype of dendritic cells, cultured 
      alone or with hUCBDSCs, was analyzed by flow cytometry. RESULTS: Levels of immune 
      molecule expression on freshly isolated hUCBDSCs were as follows: human leukocyte 
      antigen-I (HLA-I) (84.1+/-2.9%), HLA-II (1.6+/-0.3%), CD80 (0.8+/-0.1%), CD86 
      (0.8+/-0.1%), CD40 (0.6+/-0.1%), and CD40L (0.5+/-0.1%), which was not influenced 
      by cryopreservation. T-cell proliferation in the presence of hUCBDSCs was 
      significantly lower than that of positive control. The coculture led to a 10-fold 
      increase (from 1.2+/-0.3% to 12.1+/-1.4%, P<0.001) in the proportion of 
      CD4+CD25+Foxp3+ regulatory T-cells (Tregs) and a reversion of mature dendritic 
      cells, as indicated by the down-regulation of major histocompatibility complex 
      (MHC)-II molecule (49.3% vs 25.9%, P=0.001), CD80 (47.2% vs 23.3%, P=0.001), and 
      CD86 (40.6% vs 25.1%, P=0.002). When subjected to annexin V binding and propidium 
      iodide uptake assay, the hUCBDSCs did not show the ability to induce apoptosis of 
      xenogeneic T-cells. CONCLUSION: These results demonstrate low immunogenicity and 
      immunomodulation effect of the hUCBDSCs. Reversion of mature dendritic cells and 
      increase in Treg proportion, but not cell apoptosis, can possibly contribute to 
      the suppression of xenogeneic T-cell proliferation by the hUCBDSCs.
FAU - Hao, Lei
AU  - Hao L
AD  - Department of Hematology, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, PR China.
FAU - Zhang, Cheng
AU  - Zhang C
FAU - Chen, Xing-Hua
AU  - Chen XH
FAU - Zou, Zhong-Min
AU  - Zou ZM
FAU - Zhang, Xi
AU  - Zhang X
FAU - Kong, Pei-Yan
AU  - Kong PY
FAU - Liang, Xue
AU  - Liang X
FAU - Gao, Lei
AU  - Gao L
FAU - Peng, Xian-Gui
AU  - Peng XG
FAU - Sun, Ai-Hua
AU  - Sun AH
FAU - Wang, Qing-Yu
AU  - Wang QY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Croatia
TA  - Croat Med J
JT  - Croatian medical journal
JID - 9424324
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Antigens, CD/*immunology
MH  - Dendritic Cells
MH  - *Fetal Blood
MH  - Humans
MH  - Immunogenetic Phenomena
MH  - *Immunosuppression Therapy
MH  - Spleen/cytology
MH  - Stromal Cells/*immunology
PMC - PMC2728383
EDAT- 2009/08/13 09:00
MHDA- 2009/12/16 06:00
PMCR- 2009/08/01
CRDT- 2009/08/13 09:00
PHST- 2009/08/13 09:00 [entrez]
PHST- 2009/08/13 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2009/08/01 00:00 [pmc-release]
AID - CroatMedJ_50_0351 [pii]
AID - 10.3325/cmj.2009.50.351 [doi]
PST - ppublish
SO  - Croat Med J. 2009 Aug;50(4):351-60. doi: 10.3325/cmj.2009.50.351.