PMID- 19678891
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20220408
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 23
IP  - 5
DP  - 2009 Sep-Oct
TI  - Remission of histiocytic ulcerative colitis in Boxer dogs correlates with 
      eradication of invasive intramucosal Escherichia coli.
PG  - 964-9
LID - 10.1111/j.1939-1676.2009.0363.x [doi]
AB  - BACKGROUND: Historically, histiocytic ulcerative (HUC) (or granulomatous) colitis 
      of Boxer dogs was considered an idiopathic immune-mediated disease with a poor 
      prognosis. Recent reports of dramatic responses to enrofloxacin and the discovery 
      of invasive Escherichia coli within the colonic mucosa of affected Boxer dogs 
      support an infectious etiology. HYPOTHESIS: Invasive E. coli is associated with 
      colonic inflammation in Boxer dogs with HUC, and eradication of intramucosal E. 
      coli correlates with clinical and histologic remission. ANIMALS: Seven Boxer dogs 
      with HUC. METHODS: Prospective case series. Colonic biopsies were obtained at 
      initial evaluation in 7 dogs, and in 5 dogs after treatment with enrofloxacin. 
      Biopsies were evaluated by standardized histopathology, and fluorescence in situ 
      hybridization (FISH) with probes to eubacteria and E. coli. RESULTS: Intramucosal 
      E. coli was present in colonic biopsies of 7/7 Boxers with HUC. Clinical response 
      was noted in all dogs within 2 weeks of enrofloxacin (7 + or - 3.06 mg/kg q24 h, 
      for 9.5 + or - 3.98 weeks) and was sustained in 6 dogs (median disease-free 
      interval to date of 47 months, range 17-62). FISH was negative for E. coli in 4/5 
      dogs after enrofloxacin. E. coli resistant to enrofloxacin were present in the 
      FISH-positive dog that relapsed. CONCLUSIONS AND CLINICAL RELEVANCE: The 
      correlation between clinical remission and the eradication of mucosally invasive 
      E. coli during treatment with enrofloxacin supports the causal involvement of E. 
      coli in the development of HUC in susceptible Boxer dogs. A poor response to 
      enrofloxacin treatment might be due to colonization with enrofloxacin-resistant 
      E. coli.
FAU - Mansfield, C S
AU  - Mansfield CS
AD  - School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 
      6150, Australia. c.mansfield@murdoch.edu.au
FAU - James, F E
AU  - James FE
FAU - Craven, M
AU  - Craven M
FAU - Davies, D R
AU  - Davies DR
FAU - O'Hara, A J
AU  - O'Hara AJ
FAU - Nicholls, P K
AU  - Nicholls PK
FAU - Dogan, B
AU  - Dogan B
FAU - MacDonough, S P
AU  - MacDonough SP
FAU - Simpson, K W
AU  - Simpson KW
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
DEP - 20090811
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*therapeutic use
MH  - Biopsy/veterinary
MH  - Colitis, Ulcerative/drug therapy/microbiology/pathology/*veterinary
MH  - Colon/microbiology/pathology
MH  - Dog Diseases/drug therapy/*microbiology/pathology
MH  - Dogs
MH  - Enrofloxacin
MH  - Escherichia/*growth & development
MH  - Escherichia coli Infections/drug therapy/microbiology/pathology/*veterinary
MH  - Female
MH  - Fluoroquinolones/*therapeutic use
MH  - Histocytochemistry/veterinary
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Intestinal Mucosa/drug effects/microbiology/pathology
MH  - Male
MH  - Prospective Studies
EDAT- 2009/08/15 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/08/15 09:00
PHST- 2009/08/15 09:00 [entrez]
PHST- 2009/08/15 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - JVIM363 [pii]
AID - 10.1111/j.1939-1676.2009.0363.x [doi]
PST - ppublish
SO  - J Vet Intern Med. 2009 Sep-Oct;23(5):964-9. doi: 10.1111/j.1939-1676.2009.0363.x. 
      Epub 2009 Aug 11.