PMID- 19679064
OWN - NLM
STAT- MEDLINE
DCOM- 20091020
LR  - 20220317
IS  - 1877-783X (Electronic)
IS  - 1877-7821 (Linking)
VI  - 33
IP  - 2
DP  - 2009 Aug
TI  - Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 
      induced by vanillin.
PG  - 155-60
LID - 10.1016/j.canep.2009.06.003 [doi]
AB  - BACKGROUND: Vanillin is responsible for the flavor and smell of vanilla, a widely 
      used flavoring agent. Previous studies showed that vanillin could enhance the 
      repair of mutations and thus function as an anti-mutagen. However, its role in 
      cancer, a disease that is closely related to mutation has not yet been fully 
      elucidated. METHODS: Hence, this study investigated the cytolytic and cytostatic 
      properties of vanillin against HT-29, a human colorectal cancer cell line. 
      Methods used including cell viability assay, acridine orange (AO)-ethidium 
      bromide (EB) double staining cell morphological analysis, Cell cycle analysis, 
      annexin V-propidium iodide apoptosis test and 5-bromo-2-deoxyuridine 
      (BrdU)-labeling cell proliferation assay. RESULTS: Results showed that apoptosis 
      was induced by vanillin and the IC(50) for HT-29 and NIH/3T3 normal cell lines 
      were 400 microg/ml and 1000 microg/ml, respectively. Different concentrations of 
      vanillin arrest cell cycle at different checkpoints. 
      5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest 
      was achieved at lower concentration of vanillin (200 microg/ml) while cell cycle 
      analysis by flow cytometer showed that G2/M arrest occurs at higher concentration 
      of vanillin (1000 microg/ml). CONCLUSION: Cytolytic and cytostatic effects shown 
      by vanillin showed that it could be a useful colorectal cancer preventive agent. 
      Further in vivo study should be carried out to confirm that similar effects could 
      happen in animals.
FAU - Ho, Ketli
AU  - Ho K
AD  - Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, 
      Malaysia.
FAU - Yazan, Latifah Saiful
AU  - Yazan LS
FAU - Ismail, Norsharina
AU  - Ismail N
FAU - Ismail, Maznah
AU  - Ismail M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090703
PL  - Netherlands
TA  - Cancer Epidemiol
JT  - Cancer epidemiology
JID - 101508793
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Benzaldehydes)
RN  - CHI530446X (vanillin)
SB  - IM
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Benzaldehydes/*pharmacology
MH  - Cell Cycle/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Colorectal Neoplasms/*drug therapy/*pathology
MH  - Flow Cytometry
MH  - HT29 Cells
MH  - Humans
MH  - Mice
MH  - NIH 3T3 Cells
EDAT- 2009/08/15 09:00
MHDA- 2009/10/21 06:00
CRDT- 2009/08/15 09:00
PHST- 2008/09/06 00:00 [received]
PHST- 2009/05/29 00:00 [revised]
PHST- 2009/06/02 00:00 [accepted]
PHST- 2009/08/15 09:00 [entrez]
PHST- 2009/08/15 09:00 [pubmed]
PHST- 2009/10/21 06:00 [medline]
AID - S1877-7821(09)00057-5 [pii]
AID - 10.1016/j.canep.2009.06.003 [doi]
PST - ppublish
SO  - Cancer Epidemiol. 2009 Aug;33(2):155-60. doi: 10.1016/j.canep.2009.06.003. Epub 
      2009 Jul 3.