PMID- 19679959
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20191210
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 52
IP  - 3
DP  - 2009 Jul-Sep
TI  - Serological profile of HSV-2 in patients attending STI clinic: evaluation of 
      diagnostic utility of HSV-2 IgM detection.
PG  - 353-6
LID - 10.4103/0377-4929.54992 [doi]
AB  - OBJECTIVE: The present study was done to evaluate the serological profile of 
      herpes simplex virus-2 (HSV-2) among patients attending sexually transmitted 
      infections (STI) clinic and to determine the utility of detecting HSV-2 IgM 
      antibodies in such patients. A correlation of HSV-2 infection with other STI 
      including HIV has also been attempted. MATERIALS AND METHODS: Hundred consecutive 
      patients who attended STI clinic, with one or more of the complaints as 
      enunciated by WHO in syndromic approach for the diagnosis of STI, were included 
      as subjects. All subjects were screened for common STI by standard laboratory 
      procedures/ commercially available kits. HSV-1 and HSV-2 IgM antibody was 
      detected by commercially available enzyme immuno assay kit in all patient's sera. 
      Sera were also tested for other STI, namely HIV, Hepatitis B virus, Hepatitis C 
      virus and Treponema pallidum. Antigen detection for Chlamydia trachomatis was 
      done in genital swabs of all patients by Bio-Rad Chlamydia Microplate EIA 31189 
      (United States) kit. RESULTS: Thirty patients were found to have genital herpes. 
      In 17/30 (56.6%) patients, HSV-2 serology was found to correlate with the 
      clinical diagnosis. The coexistence of other infection in HSV-2 seropositive 
      patients was detected in 8/30 patients. None of the patients having concomitant 
      infections were clinically diagnosed accurately. Sensitivity, specificity, 
      positive predictive value and negative predictive value of IgM antibodies for the 
      diagnosis of genital herpes was 73.91%, 90.91%, 70.83% and 92.91% respectively. 
      CONCLUSION: HSV-2 IgM detection could only be used as a supportive test for the 
      diagnosis of genital herpes . It needs to be emphasized that the sensitivity and 
      positive predictive value scores are pointers for further improvement in the 
      commercial assay systems and a large sample size may determine the broader 
      utility of such systems.
FAU - Choudhry, Shilpee
AU  - Choudhry S
AD  - Department of Microbiology, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, Dilshad Garden, Delhi, India. shilpee_77@yahoo.com
FAU - Ramachandran, V G
AU  - Ramachandran VG
FAU - Das, Shukla
AU  - Das S
FAU - Bhattacharya, S N
AU  - Bhattacharya SN
FAU - Mogha, Narendra Singh
AU  - Mogha NS
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/*blood
MH  - Female
MH  - Herpes Genitalis/*diagnosis/virology
MH  - Herpesvirus 2, Human/*classification/*immunology
MH  - Humans
MH  - Immunoenzyme Techniques/methods
MH  - Immunoglobulin M/*blood
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
MH  - United States
MH  - Young Adult
EDAT- 2009/08/15 09:00
MHDA- 2009/10/10 06:00
CRDT- 2009/08/15 09:00
PHST- 2009/08/15 09:00 [entrez]
PHST- 2009/08/15 09:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
AID - IndianJPatholMicrobiol_2009_52_3_353_54992 [pii]
AID - 10.4103/0377-4929.54992 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2009 Jul-Sep;52(3):353-6. doi: 
      10.4103/0377-4929.54992.