PMID- 19680890
OWN - NLM
STAT- MEDLINE
DCOM- 20100514
LR  - 20191210
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Feb
TI  - Single-laboratory validation of a method for ergosterol determination in cereals.
PG  - 201-6
LID - 10.1080/02652030802399018 [doi]
AB  - Ergosterol (ERG) is a sterol produced by most fungi, but not by most plants. 
      Thus, measurement of ERG in cereals makes it possible to determine the presence 
      of fungi in cereals that can cause quality problems, such as mycotoxin 
      contamination. This study developed and performed a single-laboratory validation 
      for a method to test for ERG in various cereals. ERG was extracted by refluxing 
      samples for 1 h with methanol-sodium hydroxide. ERG was extracted from the 
      extract with hexane and then purified using a silica gel cartridge column. ERG 
      was then separated and detected by reverse-phase high-performance liquid 
      chromatography (HPLC). 'Within-day' recoveries of ERG at low levels were 92-99% 
      with relative standard deviations (RSDs) of 3.2-6.5%. 'Between-day' recoveries of 
      ERG at low levels were 97% and RSDs were 4.2-10.2%, respectively. Average 
      recoveries of ERG over the range from 1.0 to 100.0 mg kg(-1) were 81-105% and 
      RSDs were 3.9-16.3%.
FAU - Miyagawa, H
AU  - Miyagawa H
AD  - Faculty of Agriculture, Shinshu University, Kamiina, Nagano, Japan.
FAU - Umeda, M
AU  - Umeda M
FAU - Sato, T
AU  - Sato T
FAU - Bandoh, S
AU  - Bandoh S
FAU - Nakamura, S
AU  - Nakamura S
FAU - Goto, T
AU  - Goto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Mycotoxins)
RN  - Z30RAY509F (Ergosterol)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Edible Grain/*chemistry/microbiology
MH  - Ergosterol/*analysis
MH  - Food Contamination/*analysis
MH  - Japan
MH  - Mycotoxins/*analysis
MH  - Spores, Fungal/*chemistry
EDAT- 2009/08/15 09:00
MHDA- 2010/05/15 06:00
CRDT- 2009/08/15 09:00
PHST- 2009/08/15 09:00 [entrez]
PHST- 2009/08/15 09:00 [pubmed]
PHST- 2010/05/15 06:00 [medline]
AID - 905445064 [pii]
AID - 10.1080/02652030802399018 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 
      Feb;26(2):201-6. doi: 10.1080/02652030802399018.