PMID- 19682328
OWN - NLM
STAT- MEDLINE
DCOM- 20100305
LR  - 20090918
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Linking)
VI  - 10
IP  - 10
DP  - 2009 Oct
TI  - MHC II in dendritic cells is targeted to lysosomes or T cell-induced exosomes via 
      distinct multivesicular body pathways.
PG  - 1528-42
LID - 10.1111/j.1600-0854.2009.00963.x [doi]
AB  - Dendritic cells (DCs) express major histocompatibility complex class II (MHC II) 
      to present peptide antigens to T cells. In immature DCs, which bear low cell 
      surface levels of MHC II, peptide-loaded MHC II is ubiquitinated. Ubiquitination 
      drives the endocytosis and sorting of MHC II to the luminal vesicles of 
      multivesicular bodies (MVBs) for lysosomal degradation. Ubiquitination of MHC II 
      is abrogated in activated DCs, resulting in an increased cell surface expression. 
      We here provide evidence for an alternative MVB sorting mechanism for MHC II in 
      antigen-loaded DCs, which is triggered by cognately interacting antigen-specific 
      CD4+ T cells. At these conditions, DCs generate MVBs with MHC II and CD9 carrying 
      luminal vesicles that are secreted as exosomes and transferred to the interacting 
      T cells. Sorting of MHC II into exosomes was, in contrast to lysosomal targeting, 
      independent of MHC II ubiquitination but rather correlated with its incorporation 
      into CD9 containing detergent-resistant membranes. Together, these data indicate 
      two distinct MVB pathways: one for lysosomal targeting and the other for exosome 
      secretion.
FAU - Buschow, Sonja I
AU  - Buschow SI
AD  - Faculty of Veterinary Medicine, Department of Biochemistry & Cell Biology, 
      Utrecht University, The Netherlands.
FAU - Nolte-'t Hoen, Esther N M
AU  - Nolte-'t Hoen EN
FAU - van Niel, Guillaume
AU  - van Niel G
FAU - Pols, Maaike S
AU  - Pols MS
FAU - ten Broeke, Toine
AU  - ten Broeke T
FAU - Lauwen, Marjolein
AU  - Lauwen M
FAU - Ossendorp, Ferry
AU  - Ossendorp F
FAU - Melief, Cornelis J M
AU  - Melief CJ
FAU - Raposo, Graca
AU  - Raposo G
FAU - Wubbolts, Richard
AU  - Wubbolts R
FAU - Wauben, Marca H M
AU  - Wauben MH
FAU - Stoorvogel, Willem
AU  - Stoorvogel W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090714
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - CD4-Positive T-Lymphocytes/immunology/*metabolism/ultrastructure
MH  - Cell Membrane/metabolism/ultrastructure
MH  - Coculture Techniques
MH  - Dendritic Cells/immunology/*metabolism/ultrastructure
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Exosomes/immunology/*metabolism/ultrastructure
MH  - Histocompatibility Antigens Class II/genetics/*metabolism
MH  - Immunoprecipitation
MH  - Lysosomes/immunology/*metabolism/ultrastructure
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Microscopy, Immunoelectron
MH  - Multivesicular Bodies/immunology/*metabolism/ultrastructure
MH  - Protein Transport
MH  - Spleen/cytology
MH  - Ubiquitination
EDAT- 2009/08/18 09:00
MHDA- 2010/03/06 06:00
CRDT- 2009/08/18 09:00
PHST- 2009/08/18 09:00 [entrez]
PHST- 2009/08/18 09:00 [pubmed]
PHST- 2010/03/06 06:00 [medline]
AID - TRA963 [pii]
AID - 10.1111/j.1600-0854.2009.00963.x [doi]
PST - ppublish
SO  - Traffic. 2009 Oct;10(10):1528-42. doi: 10.1111/j.1600-0854.2009.00963.x. Epub 
      2009 Jul 14.