PMID- 19685316
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20090817
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 558
DP  - 2009
TI  - Analysis of Schizosaccharomyces pombe meiosis by nuclear spreading.
PG  - 15-36
LID - 10.1007/978-1-60761-103-5_2 [doi]
AB  - The fission yeast, Schizosaccharomyces pombe, much like the budding yeast, is a 
      particularly well-suited model organism for genetic research. However, the 
      miniscule size of both yeasts' nuclei has hindered their success as research 
      models for cytologists. A solution to this problem is provided by the spreading 
      of nuclei, which increases their volume and allows for a better spatial 
      resolution of nuclear contents. Here we describe nuclear spreading in fission 
      yeast. Spreading of meiotic nuclei is particularly helpful in exposing the linear 
      elements (LinEs), which are the fission yeasts' rudimentary version of the 
      synaptonemal complex. Although the LinEs' role is still not fully understood, 
      they serve as important meiotic hallmarks and their presence and morphology can 
      be used in characterizing meiotic mutants. We first describe methods to induce 
      meiosis in liquid cell cultures, then outline a method to break down cell and 
      nuclear membranes by detergent treatment to release chromatin on cytological 
      slides, and finally provide a set of protocols for analyzing these nuclei by 
      immunostaining and fluorescence in situ hybridization (FISH), and by electron 
      microscopy.
FAU - Loidl, Josef
AU  - Loidl J
AD  - Department of Chromosome Biology, Center for Molecular Biology, University of 
      Vienna, Vienna, Austria.
FAU - Lorenz, Alexander
AU  - Lorenz A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Nucleus/*genetics/physiology
MH  - Chromosomes, Fungal/chemistry/genetics
MH  - Cytogenetic Analysis/*methods
MH  - Meiosis/*genetics/physiology
MH  - Microscopy, Electron
MH  - Schizosaccharomyces/*genetics
MH  - Spores, Fungal/cytology/genetics
MH  - Staining and Labeling/methods
MH  - Tissue Fixation/*methods
RF  - 27
EDAT- 2009/08/18 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/08/18 09:00
PHST- 2009/08/18 09:00 [entrez]
PHST- 2009/08/18 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - 10.1007/978-1-60761-103-5_2 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;558:15-36. doi: 10.1007/978-1-60761-103-5_2.