PMID- 19685322
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20090817
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 558
DP  - 2009
TI  - Meiotic cytogenetics in Coprinus cinereus.
PG  - 115-27
LID - 10.1007/978-1-60761-103-5_8 [doi]
AB  - The basidiomycete fungus Coprinus cinereus has naturally synchronous meiosis and 
      is amenable to analysis using an array of well-developed genetic and molecular 
      tools. In this chapter, we explain in detail the two methods most commonly 
      employed for C. cinereus, staining of intact gill segments and chromosome 
      spreads, with an example of the application of each. We describe iron-hematoxylin 
      staining of intact gill segments for the brightfield examination of meiotic 
      progression, and the use of surface spreads and fluorescence in situ 
      hybridization (FISH) to investigate meiotic chromosome pairing. Gill segments can 
      alternatively be stained with DAPI for the determination of meiotic stage, or 
      propidium iodide for the quantitation of nuclear DNA content, and the chromosome 
      fixation and spreading techniques used for FISH are also suitable for 
      immunolocalization studies of chromosomal proteins.
FAU - Zolan, Miriam E
AU  - Zolan ME
AD  - Department of Biology, Indiana University, Bloomington, IN, USA.
FAU - Pukkila, Patricia J
AU  - Pukkila PJ
LA  - eng
GR  - GM43930/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Pairing/physiology
MH  - Chromosomes, Fungal/genetics/physiology
MH  - Coprinus/*cytology/*genetics
MH  - Cytogenetics/*methods
MH  - Meiosis/*physiology
RF  - 9
EDAT- 2009/08/18 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/08/18 09:00
PHST- 2009/08/18 09:00 [entrez]
PHST- 2009/08/18 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - 10.1007/978-1-60761-103-5_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;558:115-27. doi: 10.1007/978-1-60761-103-5_8.