PMID- 19685328
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20090817
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 558
DP  - 2009
TI  - Methods for meiotic chromosome preparation, immunofluorescence, and fluorescence 
      in situ hybridization in Daphnia pulex.
PG  - 235-49
LID - 10.1007/978-1-60761-103-5_14 [doi]
AB  - The genus Daphnia has an intriguing reproductive mode of cyclical 
      parthenogenesis. This reproductive mode has been studied for centuries, but 
      cytogenetic information is lacking due to technical limitations of classical 
      methods. We have developed methods for the preparation and examination of meiotic 
      chromosomes of Daphnia pulex from oocytes and spermatocytes. Oocyte chromosome 
      preparations are obtained by isolating individual oocytes after the release of 
      yolk granules from the ovary using pressure and capillary action. Spermatocyte 
      chromosomes are prepared using a conventional squash method. Cryosectioning is an 
      easy and fast way to prepare sections. We also illustrate the application of 
      immunofluorescence staining against alpha tubulin, as well as fluorescence in 
      situ hybridization (FISH) using the intergenic spacer of ribosomal DNA or 
      single-copy cosmid clones.
FAU - Tsuchiya, Dai
AU  - Tsuchiya D
AD  - Department of Biology, Indiana University, Bloomington, IN, USA.
FAU - Eads, Brian D
AU  - Eads BD
FAU - Zolan, Miriam E
AU  - Zolan ME
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Chromosomes/chemistry/ultrastructure
MH  - Cytological Techniques/*methods
MH  - Daphnia/*cytology/*genetics
MH  - Fluorescent Antibody Technique/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Meiosis/genetics/*physiology
RF  - 8
EDAT- 2009/08/18 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/08/18 09:00
PHST- 2009/08/18 09:00 [entrez]
PHST- 2009/08/18 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - 10.1007/978-1-60761-103-5_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;558:235-49. doi: 10.1007/978-1-60761-103-5_14.