PMID- 19685718
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20161020
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 34
IP  - 2
DP  - 2009 Apr
TI  - [Proteome analysis on the mechanism of electroacupuncture in relieving acute 
      spinal cord injury at different time courses in rats].
PG  - 75-82
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the differentially 
      expressed proteins in the spinal cord at different time courses after acute 
      spinal cord injury (ASCI) in the rat, so as to study its underlying mechanism in 
      im-proving spinal traumatic injury. METHODS: A total of 105 male SD rats were 
      randomized into normal control, model-6 h, EA-6 h, model-24 h, EA-24 h, model-48 
      h, EA-48 h groups, with 15 cases in each. ASCI model was established by using 
      modified Allen's method. EA (2 Hz, 2-5 mA) was applied to "Mingmen" (GV 4) and 
      "Dazhui" (GV 14) for 30 min. The injured spinal cord tissue (T10 -T11) was 
      collected 6 h, 24 hand 48 h after ASCI and EA treatment, weighted and stored 
      under -80 degrees D till detection. Two-dimensional gel electrophoresis (2-DE) 
      was used to separate total proteins of the spinal tissue, followed by protein 
      extraction and quantitation, 2-D gel image analysis, matrix assisted laser 
      desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS), and 
      databases-searching for identification of the differentially-expressed proteins. 
      RESULTS: A total of 10 differentially expressed proteins were identified in the 
      present study. At 6 h, compared with control group, of the 5 types of spinal 
      differential proteins, 4 were upregulated in the expression after ASCI, and the 
      rest one was downregulated; while after EA, ASCI-induced expression changes in 4 
      of the 5 differential proteins were reversed. At 24 h after ASCI, 7 types of 
      differential proteins were identified. Compared with control group, 6 
      differential proteins were upregulated, and the rest one was downregulated in 
      model group. Compared with model group, ASCI-induced expression changes in 6 of 
      the 7 differential proteins were reversed by EA. At 48 h after ASCI, a total of 8 
      types of differential proteins were identified. Compared with control group, 6 
      differential proteins were upregulated in the expression, and the rest two 
      downregulated in model group. Compared with model group, ASCI-induced expression 
      changes in 5 of the 8 differential proteins were reversed by EA. Along with the 
      increased time and treatment, 24 h vs 6 h, two more differential proteins were 
      identified, i.e., nucleoside diphosphate kinase and triosephosphate isomerase 1 
      (TPI 1). 48 h vs 24 h, 3 more differential proteins were identified, i.e., 
      dihydrolipoamide dehydrogenase, malate dehydrogenase 1, and 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH); but two proteins disappeared, 
      i.e., nucleoside diphosphate kinase, and ubiquitin-conjugating enzyme E2N. The 
      identified differential proteins involving the effects of EA in regulating 
      cellular energy metabolism, DNA repair, cellular generation, differentiation, 
      apoptosis, etc. CONCLUSION: Proteome analysis indicates that in ASCI rats, some 
      differentially expressed proteins involving energy metabolism, neuronal apoptosis 
      reduction, protein-degradation inhibition may contribute to the effect of EA in 
      repairing the traumatic spinal tissue.
FAU - Yuan, Xiao-Chen
AU  - Yuan XC
AD  - School of Acu-moxibustion, Beijing University of Chinese Medicine, Beijing 
      100029, China.
FAU - Song, Jin-Ling
AU  - Song JL
FAU - Tian, Gui-Hua
AU  - Tian GH
FAU - Shi, Su-Hua
AU  - Shi SH
FAU - Li, Zhi-Gang
AU  - Li ZG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Proteome)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Apoptosis
MH  - *Electroacupuncture
MH  - Energy Metabolism
MH  - Male
MH  - *Proteome
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/metabolism/*therapy
EDAT- 2009/08/19 09:00
MHDA- 2009/09/09 06:00
CRDT- 2009/08/19 09:00
PHST- 2009/08/19 09:00 [entrez]
PHST- 2009/08/19 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2009 Apr;34(2):75-82.