PMID- 19686312
OWN - NLM
STAT- MEDLINE
DCOM- 20100107
LR  - 20091008
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 29
IP  - 10
DP  - 2009 Nov
TI  - Porcine endogenous retrovirus released by a bioartificial liver infects primary 
      human cells.
PG  - 1553-61
LID - 10.1111/j.1478-3231.2009.02087.x [doi]
AB  - BACKGROUND: Porcine endogenous retrovirus (PERV) remains a safety risk in 
      pig-to-human xenotransplantation. There is no evidence of in vivo productive 
      infection in humans because PERV is inactivated by human serum. However, PERV can 
      infect human cell lines and human primary cells in vitro and inhibit human immune 
      functions. AIMS: We investigated the potential of primary porcine liver cells to 
      transmit PERV to primary human cells in a bioreactor-based bioartificial liver 
      (BAL). METHODS: Primary human hepatocytes, endothelial cells and the human cell 
      line HEK 293 were exposed to supernatants from BAL or from the porcine cell line 
      PK-15. PERV polymerase-specific reverse-transcriptase polymerase chain reaction 
      (RT-PCR) and PCR were used to investigate PERV transmission to human cells. An 
      assay of RT activity was used to detect the presence of retrovirus in the 
      supernatants of BAL, primary human hepatocytes and endothelial cells. RESULTS: 
      Primary human hepatocytes (hHep), endothelial cells and HEK 293 cells were 
      reproducibly infected by PERV, originating from primary porcine liver cells 
      within the BAL and from PK-15 cells. Infected cells were positive for 
      PERV-specific DNA and RNA after 8-10 days on an average, and RT activity was 
      detectable in the supernatants of infected hHep and HEK 293 cells. CONCLUSION: A 
      risk of PERV infection in human cells is documented in this study, indicating 
      that short-term contact of primary porcine liver cell supernatants with primary 
      human cells could result in PERV transmission.
FAU - Fruhauf, Jan-Henning
AU  - Fruhauf JH
AD  - Department of Cell Techniques and Applied Stem Cell Biology, 
      Biomedical-Biotechnological Center (BBZ), Leipzig, Germany.
FAU - Mertsching, Heike
AU  - Mertsching H
FAU - Giri, Shibashish
AU  - Giri S
FAU - Fruhauf, Nils Roman
AU  - Fruhauf NR
FAU - Bader, Augustinus
AU  - Bader A
LA  - eng
PT  - Journal Article
DEP - 20090814
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Endogenous Retroviruses/*physiology
MH  - Endothelial Cells/virology
MH  - Hepatocytes/virology
MH  - Humans
MH  - Liver, Artificial/*virology
MH  - Swine/*virology
EDAT- 2009/08/19 09:00
MHDA- 2010/01/08 06:00
CRDT- 2009/08/19 09:00
PHST- 2009/08/19 09:00 [entrez]
PHST- 2009/08/19 09:00 [pubmed]
PHST- 2010/01/08 06:00 [medline]
AID - LIV2087 [pii]
AID - 10.1111/j.1478-3231.2009.02087.x [doi]
PST - ppublish
SO  - Liver Int. 2009 Nov;29(10):1553-61. doi: 10.1111/j.1478-3231.2009.02087.x. Epub 
      2009 Aug 14.