PMID- 19686921
OWN - NLM
STAT- MEDLINE
DCOM- 20090901
LR  - 20161125
IS  - 1531-5053 (Electronic)
IS  - 0278-2391 (Linking)
VI  - 67
IP  - 9
DP  - 2009 Sep
TI  - Effect of platelet-rich plasma and latissimus dorsi muscle flap on osteogenesis 
      and vascularization of tissue-engineered bone in dogs.
PG  - 1850-8
LID - 10.1016/j.joms.2009.04.029 [doi]
AB  - PURPOSE: The present study evaluated the effects of platelet-rich plasma (PRP) 
      and the latissimus dorsi muscle flap on osteogenesis and vascularization of 
      tissue-engineered bone. MATERIALS AND METHODS: Bone marrow stromal cells (BMSCs) 
      were subcultured, and PRP was obtained from the same dogs. Demineralized bone 
      matrix (DBM) was prepared from homologous bone. The complexes of DBM/BMSCs/PRP 
      were implanted into areas A and B on the left side of the dogs' backs; complexes 
      of DBM/BMSCs without PRP were implanted in areas C and D on the right side of the 
      same dog. The implants in areas A and C were wrapped with a latissimus dorsi 
      muscle flap, and the implants in areas B and D were wrapped with inferior fascia. 
      At 4, 8, and 12 weeks later, the implants were removed for evaluation. RESULTS: 
      The radiographic evaluation, descriptive histologic analysis, and histologic 
      quantitative analysis showed that the PRP/BMSCs/DBM complex was better than the 
      BMSCs/DBM complex in both vascularization and osteogenesis of the ectopic 
      tissue-engineered bones, and the complex wrapped with the latissimus dorsi muscle 
      flap was better than that packed with superficial fascia without blood vessels. 
      CONCLUSIONS: The PRP and blood vessels in the latissimus dorsi muscle could 
      cooperatively promote osteogenesis and vascularization in tissue-engineered bone.
FAU - Li, Ning-Yi
AU  - Li NY
AD  - Department of Oral and Maxillofacial Surgery, Qingdao University Medical School 
      Hospital, Qingdao, China. ningyili342@163.com
FAU - Yuan, Rong-Tao
AU  - Yuan RT
FAU - Chen, Tao
AU  - Chen T
FAU - Chen, Li-Qiang
AU  - Chen LQ
FAU - Jin, Xiao-Ming
AU  - Jin XM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Oral Maxillofac Surg
JT  - Journal of oral and maxillofacial surgery : official journal of the American 
      Association of Oral and Maxillofacial Surgeons
JID - 8206428
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Bone Matrix/transplantation
MH  - Dogs
MH  - Fasciotomy
MH  - Muscle, Skeletal/*surgery
MH  - *Neovascularization, Physiologic
MH  - *Osteogenesis
MH  - *Platelet-Rich Plasma
MH  - Stromal Cells/transplantation
MH  - *Surgical Flaps
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
EDAT- 2009/08/19 09:00
MHDA- 2009/09/02 06:00
CRDT- 2009/08/19 09:00
PHST- 2008/04/29 00:00 [received]
PHST- 2009/01/30 00:00 [revised]
PHST- 2009/04/19 00:00 [accepted]
PHST- 2009/08/19 09:00 [entrez]
PHST- 2009/08/19 09:00 [pubmed]
PHST- 2009/09/02 06:00 [medline]
AID - S0278-2391(09)00529-1 [pii]
AID - 10.1016/j.joms.2009.04.029 [doi]
PST - ppublish
SO  - J Oral Maxillofac Surg. 2009 Sep;67(9):1850-8. doi: 10.1016/j.joms.2009.04.029.