PMID- 19687469
OWN - NLM
STAT- MEDLINE
DCOM- 20091221
LR  - 20240408
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 57
IP  - 12
DP  - 2009 Dec
TI  - Application of a novel method of double APAAP staining with subsequent 
      quantitative image analysis to the examination of integrin expression in 
      undifferentiated-type gastric carcinomas.
PG  - 1183-93
LID - 10.1369/jhc.2009.954263 [doi]
AB  - In undifferentiated-type gastric carcinoma (UGC), recognition of cancer cells is 
      not easy, which has hampered its precise phenotypic analysis. To examine 
      alterations of the integrin phenotype during the progression of UGC, we used 
      double alkaline phosphatase anti-alkaline phosphatase staining and computer-aided 
      image analyses for the expression of alpha1, alpha2, alpha3, alpha5, alpha6, 
      alphaV, beta1, and beta4 integrin subunits and alphaVbeta3, alphaVbeta5, and 
      alphaVbeta6 integrins in cytokeratin-positive cells in the mucosal, the 
      submucosal, and the deeper parts of 10 early and 17 advanced UGCs, their 
      non-neoplastic counterparts, and 9 lymph node (LN) metastases. We revealed 
      declining expression of epithelial integrin subunits (alpha2, alpha3, alpha6, 
      beta4) and increasing expression of mesenchymal integrin subunits (alpha1, 
      alpha5) as the tumor invaded deeper, reflecting gradual epithelial-to-mesenchymal 
      transition of the integrin phenotype during tumor invasion. Enhanced expression 
      of the alphaV integrin subunit and alphaVbeta3 and alphaVbeta5 integrins 
      correlated with tumor invasion, and that of alphaVbeta6 integrins with LN 
      metastasis. Our results have demonstrated that the method we introduced is 
      suitable for analysis of dynamic alterations of the integrin repertoire in UGC 
      progression.
FAU - Yanchenko, Natalia
AU  - Yanchenko N
AD  - Department of Pathology, Shiga University of Medical Science, Seta-Tsukinowa cho, 
      Otsu-shi 520-2192, Japan. natalia.yanchenko@gmail.com
FAU - Sugihara, Hiroyuki
AU  - Sugihara H
FAU - Hattori, Takanori
AU  - Hattori T
LA  - eng
PT  - Journal Article
DEP - 20090817
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Integrin alphaV)
RN  - 0 (Integrins)
RN  - 0 (Protein Subunits)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/immunology/*metabolism
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/*metabolism
MH  - *Cell Differentiation
MH  - Epithelium/metabolism/pathology
MH  - Gastric Mucosa/cytology/metabolism
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Integrin alphaV/metabolism
MH  - Integrins/*metabolism
MH  - Mesoderm/metabolism/pathology
MH  - Mice
MH  - Protein Subunits/metabolism
MH  - Staining and Labeling/*methods
MH  - Stomach Neoplasms/genetics/metabolism/*pathology
PMC - PMC2778091
EDAT- 2009/08/19 09:00
MHDA- 2009/12/22 06:00
PMCR- 2010/12/01
CRDT- 2009/08/19 09:00
PHST- 2009/08/19 09:00 [entrez]
PHST- 2009/08/19 09:00 [pubmed]
PHST- 2009/12/22 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - jhc.2009.954263 [pii]
AID - 954263 [pii]
AID - 10.1369/jhc.2009.954263 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2009 Dec;57(12):1183-93. doi: 10.1369/jhc.2009.954263. Epub 
      2009 Aug 17.