PMID- 1968926
OWN - NLM
STAT- MEDLINE
DCOM- 19900416
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 144
IP  - 6
DP  - 1990 Mar 15
TI  - Interactions between a lymphoma membrane-associated guanosine 
      5'-triphosphate-binding protein and the cytoskeleton during receptor patching and 
      capping.
PG  - 2242-52
AB  - In this study we have used several complementary techniques to isolate and 
      characterize a lymphoma membrane-associated 41-kDa protein that shares a number 
      of structural and functional similarities with the alpha i subunit of the 
      guanosine 5'-triphosphate (GTP)-binding protein (e.g., Gi alpha-like protein). In 
      addition, using permeabilized lymphoma cells, we have found that: 1) GTP or 
      GTP-tau-S augments, and pertussis toxin inhibits, phospholipase C (PLC) activity 
      and receptor capping; and 2) the addition of lymphoma 41-kDa Gi alpha-like 
      protein stimulates PLC activity and receptor patching/capping, and reverses the 
      inhibitory effect of pertussis toxin on both activity and receptor 
      patching/capping. Additional cytochemical and biochemical data indicate that the 
      lymphoma 41-kDa protein is closely associated with several cytoskeletal proteins 
      (e.g., actin, myosin, and fodrin) all of which colocalize under receptor cap 
      structures. Furthermore, both the 41-kDa-mediated phospholipase C activity and 
      receptor patching/capping are inhibited by cytochalasin D (a microfilament 
      disrupting drug) and W-7 drug (a calmodulin inhibitor). Together, these data 
      provide strong evidence for a functional association between the lymphoma 
      membrane cytoskeleton and the 41-kDa (Gi alpha-like) protein. Specifically, this 
      association appears to be required for the activation of phospholipase C that 
      results in inositol triphosphate production, subsequent internal Ca2+ release, 
      and finally surface receptor patching and capping.
FAU - Bourguignon, L Y
AU  - Bourguignon LY
AD  - Department of Cell Biology and Anatomy, School of Medicine, University of Miami, 
      FL 33101.
FAU - Walker, G
AU  - Walker G
FAU - Huang, H S
AU  - Huang HS
LA  - eng
GR  - GM 36353/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Surface)
RN  - 0 (Membrane Proteins)
RN  - 0 (Sulfonamides)
RN  - 0 (Thy-1 Antigens)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 22144-77-0 (Cytochalasin D)
RN  - 65595-90-6 (W 7)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Cell Line
MH  - Cytochalasin D/pharmacology
MH  - Cytoskeleton/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Guanosine Triphosphate/pharmacology
MH  - *Immunologic Capping/drug effects
MH  - Lymphoma/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Molecular Weight
MH  - Peptide Mapping
MH  - Pertussis Toxin
MH  - *Receptor Aggregation/drug effects
MH  - Sulfonamides/pharmacology
MH  - Thy-1 Antigens
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/metabolism
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1990/03/15 00:00
MHDA- 1990/03/15 00:01
CRDT- 1990/03/15 00:00
PHST- 1990/03/15 00:00 [pubmed]
PHST- 1990/03/15 00:01 [medline]
PHST- 1990/03/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1990 Mar 15;144(6):2242-52.