PMID- 19689785
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20090819
IS  - 1435-8603 (Print)
IS  - 1435-8603 (Linking)
VI  - 11
IP  - 5
DP  - 2009 Sep
TI  - A contribution to characterisation of genetic variation in some natural Polish 
      populations of Elymus repens (L.) Gould and Elymus hispidus (Opiz) Melderis 
      (Poaceae) as revealed by RAPD markers.
PG  - 766-73
LID - 10.1111/j.1438-8677.2008.00171.x [doi]
AB  - To determine the relative importance of clonal growth and sexual reproduction, 
      the Randomly Amplified Polymorphic DNA (RAPD) method was used to study genetic 
      diversity and clonal structure of six populations of Elymus repens and four 
      populations of Elymus hispidus from Poland. These outbreeding species are 
      virtually self-sterile and form widely spreading and long-lived rhizomes. Using 
      12 primers, a total of 150 unambiguous RAPD fragments were amplified and scored. 
      Results of AMOVA showed no significant genetic distinction between 
      morphologically distinguished varieties of E. repens and E. hispidus. E. repens 
      had slightly higher intra-specific genetic polymorphism than E. hispidus; the 
      percentage of polymorphic bands per population ranged from 38 to 49 and from 19 
      to 38 respectively. Clonal diversity measured using the Simpson diversity index 
      (D) indicated different contributions of clonal reproduction in particular 
      populations of E. repens (D: 0.20-0.72). Populations of E. hispidus were 
      dominated by one or a few clones, which were generally restricted to a single 
      population (D: 0.00-0.22). RAPD revealed that most genetic diversity resided 
      within populations of the two studied species, suggesting that, despite their 
      clonal character, propagation by seeds contributes considerably to reproduction 
      of E. repens and E. hispidus.
FAU - Szczepaniak, M
AU  - Szczepaniak M
AD  - Department of Vascular Plant Systematics, Polish Academy of Sciences, W. Szafer 
      Institute of Botany, Krakow, Poland.
FAU - Bieniek, W
AU  - Bieniek W
FAU - Boron, P
AU  - Boron P
FAU - Szklarczyk, M
AU  - Szklarczyk M
FAU - Mizianty, M
AU  - Mizianty M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Biol (Stuttg)
JT  - Plant biology (Stuttgart, Germany)
JID - 101148926
SB  - IM
MH  - Elymus/*genetics/growth & development
MH  - *Genetic Variation
MH  - Poland
MH  - Random Amplified Polymorphic DNA Technique
MH  - *Reproduction, Asexual
EDAT- 2009/08/20 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/08/20 09:00
PHST- 2009/08/20 09:00 [entrez]
PHST- 2009/08/20 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - PLB171 [pii]
AID - 10.1111/j.1438-8677.2008.00171.x [doi]
PST - ppublish
SO  - Plant Biol (Stuttg). 2009 Sep;11(5):766-73. doi: 
      10.1111/j.1438-8677.2008.00171.x.