PMID- 19691323
OWN - NLM
STAT- MEDLINE
DCOM- 20091130
LR  - 20161125
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 17
DP  - 2009 Sep 9
TI  - In vitro inhibitory effect of apple peel extract on the growth of Helicobacter 
      pylori and respiratory burst induced on human neutrophils.
PG  - 7743-9
LID - 10.1021/jf9006592 [doi]
AB  - In the present work, the in vitro effect of a standardized extract of apple peel 
      APPE (60% of total polyphenols; 58% of flavonoids; 30% of flavan-3-ols and 
      procyanidins) was evaluated with regard to the viability of Helicobacter pylori. 
      The cytotoxic effect of APPE on H. pylori was also evaluated through the 
      resazurin assay and ATP level determination. In both assays, APPE showed an early 
      cytotoxic effect, which was both concentration and time-dependent. Additionally, 
      the effect of APPE on the intra and extracellular production of reactive oxygen 
      species (ROS) was evaluated in human neutrophils stimulated by H. pylori, phorbol 
      myristate acetate (PMA), and formyl-methionyl-leucyl-phenylalanine (fMLP). The 
      extracellular and intracellular production of ROS was evaluated through 
      chemiluminiscence with the isoluminol-horseradish peroxidase (HRP) and 
      luminol-superoxide dismutase (SOD)-catalase systems, respectively. APPE showed an 
      inhibiting effect on the multiplication of two H. pylori strains (ATCC 43504 and 
      TX136) with a miminnum inhibitory concentration (MIC) value of 112.5 microg 
      gallic acid equivalent (GAE)/mL. APPE inhibited the respiratory burst of 
      neutrophils induced by H. pylori, PMA, and fMLP in concentration-dependent form. 
      Interestingly, this effect was observed on both the interior and exterior of the 
      neutrophil. This result suggests that apple peel polyphenols have an attenuating 
      effect on the damage to gastric mucosa caused by neutrophil generated ROS and, 
      particularly, when H. pylori displays its evasion mechanisms.
FAU - Pastene, Edgar
AU  - Pastene E
AD  - Laboratory of Antioxidants, Institute of Nutrition and Food Technology, 
      Universidad de Chile, Santiago, Chile. edgar.pastene@gmail.com
FAU - Speisky, Hernan
AU  - Speisky H
FAU - Troncoso, Miriam
AU  - Troncoso M
FAU - Alarcon, Julio
AU  - Alarcon J
FAU - Figueroa, Guillermo
AU  - Figueroa G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphenols)
RN  - 0 (Proanthocyanidins)
RN  - 35HDD3NRIE (flavan-3-ol)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adenosine Triphosphate/analysis
MH  - Flavonoids/analysis
MH  - Fruit/*chemistry
MH  - Helicobacter pylori/chemistry/*drug effects/growth & development
MH  - Humans
MH  - Malus/*chemistry
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/physiology
MH  - Phenols/analysis
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Polyphenols
MH  - Proanthocyanidins/analysis
MH  - Respiratory Burst/*drug effects
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2009/08/21 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/21 09:00
PHST- 2009/08/21 09:00 [entrez]
PHST- 2009/08/21 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1021/jf9006592 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Sep 9;57(17):7743-9. doi: 10.1021/jf9006592.