PMID- 19692468
OWN - NLM
STAT- MEDLINE
DCOM- 20091123
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 21
DP  - 2009 Nov
TI  - An amphipathic alpha-helix at the C terminus of hepatitis C virus nonstructural 
      protein 4B mediates membrane association.
PG  - 11378-84
LID - 10.1128/JVI.01122-09 [doi]
AB  - Nonstructural protein 4B (NS4B) plays an essential role in the formation of the 
      hepatitis C virus (HCV) replication complex. It is an integral membrane protein 
      that has been only poorly characterized to date. It is believed to comprise a 
      cytosolic N-terminal part, a central part harboring four transmembrane passages, 
      and a cytosolic C-terminal part. Here, we describe an amphipathic alpha-helix at 
      the C terminus of NS4B (amino acid residues 229 to 253) that mediates membrane 
      association and is involved in the formation of a functional HCV replication 
      complex.
FAU - Gouttenoire, Jerome
AU  - Gouttenoire J
AD  - Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire 
      Vaudois, BU44/07/2421, Rue du Bugnon 44, CH-1011 Lausanne, Switzerland.
FAU - Montserret, Roland
AU  - Montserret R
FAU - Kennel, Audrey
AU  - Kennel A
FAU - Penin, Francois
AU  - Penin F
FAU - Moradpour, Darius
AU  - Moradpour D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090819
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Hepacivirus/*metabolism
MH  - Humans
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - *Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Viral Nonstructural Proteins/*chemistry/genetics/metabolism
MH  - *Virus Internalization
MH  - Virus Replication
PMC - PMC2772773
EDAT- 2009/08/21 09:00
MHDA- 2009/12/16 06:00
PMCR- 2010/05/01
CRDT- 2009/08/21 09:00
PHST- 2009/08/21 09:00 [entrez]
PHST- 2009/08/21 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - JVI.01122-09 [pii]
AID - 1122-09 [pii]
AID - 10.1128/JVI.01122-09 [doi]
PST - ppublish
SO  - J Virol. 2009 Nov;83(21):11378-84. doi: 10.1128/JVI.01122-09. Epub 2009 Aug 19.