PMID- 19692747
OWN - NLM
STAT- MEDLINE
DCOM- 20091123
LR  - 20130418
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 129
IP  - 6
DP  - 2009 Jun
TI  - Effect of gonadotropins on chromosome aneuploidy, chromosome mosaicism & sex 
      ratio in mouse preimplantation embryos.
PG  - 669-75
AB  - BACKGROUND & OBJECTIVE: There are potential risks of major birth defect in IVF 
      (in vitro fertilization) pregnancy as well as IVF-ICSI (intra cytoplasmic sperm 
      injection) pregnancies in comparison with naturally conceived human pregnancies. 
      This increase risk could be due to either gonadotropins used for ovarian 
      stimulation or in vitro culture conditions or multiple pregnancy or combinations 
      of all the factors. The effects of gonadotropins on chromosome aneuploidy, 
      chromosome mosaicism and sex ratio on mouse preimplantation embryos were 
      evaluated through the use of fluorescence in situ hybridization (FISH). METHODS: 
      The study material consisted of 111 preimplantation mouse embryos (2-16 cell 
      stage) in control group and 405 preimplantation mouse embryos in gonadotropin 
      stimulated group from genetically identical Swiss Albino young (6-8 wk) mouse 
      kept in a similar environmental conditions. The study was designed to investigate 
      effect of gonadotropins on chromosome aneuploidy, chromosome mosaicism and sex 
      ratio through the use of FISH technique using chromosome X, Y and 19 probes. All 
      blastomeres of embryos in both groups were assessed. RESULTS: Interpretable FISH 
      results were obtained in 66 embryos in control group and 128 embryos in 
      gonadotropin stimulated group. There was no excess of chromosome aneuploidy (only 
      one case of sex chromosome trisomy in study group; 19, 19, X, Y, Y) or chromosome 
      mosaicism or deviations in sex ratio between the two groups. However, deviation 
      (1.36 M: 1 F in control group & 1.25 M : 1 F in study group) was seen from 
      expected sex ratio (1 M : 1 F) i.e., skewed sex ratio in both the groups. 
      INTERPRETATION & CONCLUSION: Our results showed that gonadotropins used for 
      ovarian stimulation had no effects in causing increase in chromosome X, Y, 19 
      aneuploidy and mosaicism and skewing of sex ratio in mouse model. A large scale 
      study with more FISH probes on a larger sample size need to be done to confirm 
      the findings.
FAU - Fauzdar, Ashish
AU  - Fauzdar A
AD  - Department of Reproductive Biology, All India Institute of Medical Sciences, New 
      Delhi, India.
FAU - Halder, Ashutosh
AU  - Halder A
FAU - Kumar, Anand
AU  - Kumar A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Gonadotropins)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - *Blastocyst/drug effects
MH  - Chromosomes, Mammalian/drug effects/genetics
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - Gonadotropins/*pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mosaicism/*drug effects
MH  - Ovulation Induction/methods
MH  - Pregnancy
MH  - *Sex Ratio
EDAT- 2009/08/21 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/21 09:00
PHST- 2009/08/21 09:00 [entrez]
PHST- 2009/08/21 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PST - ppublish
SO  - Indian J Med Res. 2009 Jun;129(6):669-75.