PMID- 19694218
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20170306
VI  - 119
IP  - 6
DP  - 2009 Jun
TI  - Usefulness of classic cytogenetic testing compared to fluorescence in situ 
      hybridization in genetic diagnosis of 58 patients with myelodysplastic syndromes.
PG  - 366-72
AB  - INTRODUCTION: Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal 
      diseases of pluripotent hematopoietic stem or progenitor cells. MDS are 
      characterized by ineffective hematopoiesis, increased apoptosis, peripheral blood 
      cytopenias, and propensity to evolve into acute myelogenous leukemia. OBJECTIVES: 
      The aim of our investigation was to compare the usefulness of classic 
      cytogenetics and fluorescence in situ hybridization (FISH) to detect chromosome 
      aberrations in myelodysplastic syndromes. PATIENTS AND METHODS: The study was 
      carried out in a group of 58 patients with MDS. G-banding using trypsin and 
      Giemsa (GTG banding) and FISH with a panel of five molecular probes for 
      aberrations with prognostic significance in MDS (cen7/8, 5q31, 7q22/q35, 17p13, 
      20q13.3) were performed on bone marrow cells. RESULTS: The use of GTG technique 
      allowed to detect chromosome aberrations in 25 (43.1%) subjects. However, the 
      additional use of FISH showed the presence of aberrations also in additional 10 
      (17.2%) patients, which shifted 11 patients from one cytogenetic category to 
      another. CONCLUSIONS: The use of FISH with MDS probe panel beside classic 
      cytogenetics improves detection of chromosome aberrations, and also 
      stratification of MDS patients to prognostic groups. Both methods should be used 
      simultaneously in every genetically diagnosed MDS patient.
FAU - Skonieczka, Katarzyna
AU  - Skonieczka K
AD  - Department of Clinical Genetics, Collegium Medicum, Nicolaus Copernicus 
      University, Bydgoszcz, Poland.
FAU - Duszenko, Ewa
AU  - Duszenko E
FAU - Wyrowinska, Elzbieta
AU  - Wyrowinska E
FAU - Haus, Olga
AU  - Haus O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Pol Arch Med Wewn
JT  - Polskie Archiwum Medycyny Wewnetrznej
JID - 0401225
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/*pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/*pathology
MH  - Reference Values
EDAT- 2009/08/22 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/08/22 09:00
PHST- 2009/08/22 09:00 [entrez]
PHST- 2009/08/22 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
PST - ppublish
SO  - Pol Arch Med Wewn. 2009 Jun;119(6):366-72.