PMID- 19694417
OWN - NLM
STAT- MEDLINE
DCOM- 20091203
LR  - 20161125
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 25
IP  - 19
DP  - 2009 Oct 6
TI  - General strategy for designing functionalized magnetic microspheres for different 
      bioapplications.
PG  - 11657-63
LID - 10.1021/la901258p [doi]
AB  - Surface functionalization and water solubility of magnetic nanoparticles are 
      crucial for bioapplication. Here, we describe a synthetic approach for direct 
      preparation of a wide range of functionalized and hydrophilic magnetic polymer 
      particles (MPPs) that is both simple and general and involves using different 
      polymers as the source of functional groups. This simple strategy of changing the 
      polymer used in the reaction can give rise to a wide variety of hydrophilic MPPs 
      with a high number of functional groups. For the purpose of bioapplication, we 
      synthesized three types of MPPs with typical functional groups, such as hydroxyl 
      groups (-OH), amino groups (-NH2), and carboxyl groups (-COOH), and further 
      characterized these MPPs by transmission electronic microscopy (TEM), scanning 
      electronic microscopy (SEM), thermogravimetric analysis (TGA), X-ray powder 
      diffraction (XRD), Raman spectroscopy, and Fourier transform infrared (FTIR) 
      spectroscopy. The magnetic saturation of the MPPs was also studied and was 
      adequate for most bioapplications. MPPs were shown to have good biocompatibility 
      using cell proliferation and apoptosis assays. Two types of MPPs with different 
      functional groups were used successfully for intracellular imaging and antibody 
      purification. Our results demonstrate that this simple and general synthesis 
      strategy has potential for designing hydrophilic magnetic nanoparticles with 
      multifunctionalities that cater for a range of bioapplications.
FAU - Huang, Xinglu
AU  - Huang X
AD  - Laboratory of Controllable Preparation and Application of Nanomaterials, 
      Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 
      Beijing 100190, China.
FAU - Zhuang, Jie
AU  - Zhuang J
FAU - Chen, Dong
AU  - Chen D
FAU - Liu, Huiyu
AU  - Liu H
FAU - Tang, Fangqiong
AU  - Tang F
FAU - Yan, Xiyun
AU  - Yan X
FAU - Meng, Xianwei
AU  - Meng X
FAU - Zhang, Lin
AU  - Zhang L
FAU - Ren, Jun
AU  - Ren J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Acrylic Resins)
RN  - 0 (Antibodies)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dextrans)
RN  - 4Q93RCW27E (carbopol 940)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Acrylic Resins/*chemistry/metabolism/pharmacology
MH  - Animals
MH  - Antibodies/chemistry/isolation & purification/metabolism
MH  - Apoptosis/drug effects
MH  - Biocompatible Materials/*chemistry/metabolism/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Chitosan/*chemistry/metabolism/pharmacology
MH  - Dextrans/*chemistry/metabolism/pharmacology
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - *Magnetics
MH  - Mice
MH  - *Microspheres
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Spectrum Analysis, Raman
MH  - Surface Properties
MH  - X-Ray Diffraction
EDAT- 2009/08/22 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/22 09:00
PHST- 2009/08/22 09:00 [entrez]
PHST- 2009/08/22 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1021/la901258p [doi]
PST - ppublish
SO  - Langmuir. 2009 Oct 6;25(19):11657-63. doi: 10.1021/la901258p.