PMID- 19694639
OWN - NLM
STAT- MEDLINE
DCOM- 20090914
LR  - 20240320
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 62
IP  - 3
DP  - 2009 Sep
TI  - H3N2 influenza A virus replicates in immortalized human first trimester 
      trophoblast cell lines and induces their rapid apoptosis.
PG  - 139-46
LID - 10.1111/j.1600-0897.2009.00723.x [doi]
AB  - PROBLEM: Epidemiological data suggested that pandemic influenza increased the 
      risks of spontaneous abortion and premature labor, while seasonal influenza also 
      increased the risk of schizophrenia in adolescence. However, their pathogenesis 
      is so far unknown. METHOD OF STUDY: The first trimester trophoblast cell lines, 
      namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus 
      (H3N2). At indicated time points, cells were examined for expression of influenza 
      proteins. Viral replication in culture media, apoptosis and the expression of 
      human leukocyte antigen (HLA)-G were also examined. RESULTS: Intracellular 
      localization of viral proteins was observed. Twenty-four hours after inoculation, 
      virus was detected in culture media while most cells fell into apoptosis. During 
      apoptosis, expression of HLA-G was unchanged. CONCLUSION: We revealed replication 
      of low pathogenic influenza virus in the first trimester trophoblast cell lines. 
      Placental damages are likely to be induced by direct cytopathic effects of 
      influenza virus and subsequent apoptosis rather than down regulation of HLA-G 
      expression and subsequent rejection by maternal immune system.
FAU - Trinh, Quang Duy
AU  - Trinh QD
AD  - Division of Microbiology, Department of Pathology and Microbiology, Nihon 
      University School of Medicine, Itabashi-ku, Tokyo, Japan.
FAU - Izumi, Yasuyuki
AU  - Izumi Y
FAU - Komine-Aizawa, Shihoko
AU  - Komine-Aizawa S
FAU - Shibata, Toshikatsu
AU  - Shibata T
FAU - Shimotai, Yoshitaka
AU  - Shimotai Y
FAU - Kuroda, Kazumichi
AU  - Kuroda K
FAU - Mizuguchi, Masashi
AU  - Mizuguchi M
FAU - Ushijima, Hiroshi
AU  - Ushijima H
FAU - Mor, Gil
AU  - Mor G
FAU - Hayakawa, Satoshi
AU  - Hayakawa S
LA  - eng
GR  - P01 HD054713/HD/NICHD NIH HHS/United States
GR  - P01 HD054713-03/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antigens, Viral)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/immunology
MH  - *Apoptosis
MH  - Cell Line
MH  - Dogs
MH  - Female
MH  - HLA Antigens/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Influenza A Virus, H3N2 Subtype/*physiology
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - RNA, Viral/genetics
MH  - Trophoblasts/*cytology/immunology/*virology
MH  - *Virus Replication
PMC - PMC3025808
MID - NIHMS263792
EDAT- 2009/08/22 09:00
MHDA- 2009/09/15 06:00
PMCR- 2011/01/24
CRDT- 2009/08/22 09:00
PHST- 2009/08/22 09:00 [entrez]
PHST- 2009/08/22 09:00 [pubmed]
PHST- 2009/09/15 06:00 [medline]
PHST- 2011/01/24 00:00 [pmc-release]
AID - AJI723 [pii]
AID - 10.1111/j.1600-0897.2009.00723.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2009 Sep;62(3):139-46. doi: 
      10.1111/j.1600-0897.2009.00723.x.