PMID- 19694763
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20191210
IS  - 1469-0691 (Electronic)
IS  - 1198-743X (Linking)
VI  - 16
IP  - 6
DP  - 2010 Jun
TI  - Fluorescence in situ hybridization to improve the diagnosis of endocarditis: a 
      pilot study.
PG  - 767-73
LID - 10.1111/j.1469-0691.2009.02936.x [doi]
AB  - Infective endocarditis is a rare but life-threatening disease associated with 
      high mortality. Early diagnosis of the causative microorganism is critical to 
      patient outcome. However, conventional diagnostic methods are often 
      unsatisfactory in achieving this goal. As a proof of concept, we applied 
      fluorescence in situ hybridization (FISH) for detection and identification of 
      bacteria in histological sections of heart valves. Biopsy specimens from 54 
      suspected endocarditis patients were obtained during valve surgery and analysed 
      via FISH. Specimens were screened with a probe panel that identifies the most 
      common bacteria implicated in endocarditis. Results were compared with those of 
      culture-based diagnostics and clinical data. Discrepant results were subjected to 
      comparative sequence analysis of PCR-amplified 16S rRNA genes. FISH detected 
      bacteria in 26 of the 54 heart valves. FISH allowed successful diagnosis of 
      infective endocarditis in five of 13 blood culture-negative cases and in 11 of 37 
      valve culture-negative cases, showing the bacteria within their histological 
      context. This technique allows the simultaneous detection and identification of 
      microorganisms at the species or genus level directly from heart valves and might 
      be a valuable tool for diagnosis of endocarditis.
FAU - Mallmann, C
AU  - Mallmann C
AD  - Institut fur Mikrobiologie und Hygiene, Charite- Universitatsmedizin Berlin, 
      Germany.
FAU - Siemoneit, S
AU  - Siemoneit S
FAU - Schmiedel, D
AU  - Schmiedel D
FAU - Petrich, A
AU  - Petrich A
FAU - Gescher, D M
AU  - Gescher DM
FAU - Halle, E
AU  - Halle E
FAU - Musci, M
AU  - Musci M
FAU - Hetzer, R
AU  - Hetzer R
FAU - Gobel, U B
AU  - Gobel UB
FAU - Moter, A
AU  - Moter A
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090820
PL  - England
TA  - Clin Microbiol Infect
JT  - Clinical microbiology and infection : the official publication of the European 
      Society of Clinical Microbiology and Infectious Diseases
JID - 9516420
SB  - IM
MH  - Bacteria/*classification/genetics/*isolation & purification
MH  - Bacteriological Techniques/*methods
MH  - Biopsy
MH  - Endocarditis, Bacterial/*diagnosis
MH  - Heart Valves/microbiology
MH  - Histocytochemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Pilot Projects
MH  - Sensitivity and Specificity
EDAT- 2009/08/22 09:00
MHDA- 2011/01/19 06:00
CRDT- 2009/08/22 09:00
PHST- 2009/08/22 09:00 [entrez]
PHST- 2009/08/22 09:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - S1198-743X(14)61726-2 [pii]
AID - 10.1111/j.1469-0691.2009.02936.x [doi]
PST - ppublish
SO  - Clin Microbiol Infect. 2010 Jun;16(6):767-73. doi: 
      10.1111/j.1469-0691.2009.02936.x. Epub 2009 Aug 20.