PMID- 19696080
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20211020
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 8
IP  - 11
DP  - 2009 Nov
TI  - Recombinant surface proteomics as a tool to analyze humoral immune responses in 
      bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
PG  - 2544-54
LID - 10.1074/mcp.M900009-MCP200 [doi]
AB  - A systematic approach to characterize the surface proteome of Mycoplasma mycoides 
      subspecies mycoides small colony type (M. mycoides SC), the causative agent of 
      contagious bovine pleuropneumonia (CBPP) in cattle, is presented. Humoral immune 
      responses in 242 CBPP-affected cattle and controls were monitored against 
      one-third of the surface proteins of M. mycoides SC in a high throughput magnetic 
      bead-based assay. Initially, 64 surface proteins were selected from the genome 
      sequence of M. mycoides SC and expressed as recombinant proteins in Escherichia 
      coli. Binding of antibodies to each individual protein could then be analyzed 
      simultaneously in minute sample volumes with the Luminex suspension array 
      technology. The assay was optimized on Namibian CBPP-positive sera and Swedish 
      negative controls to allow detection and 20-fold mean signal separation between 
      CBPP-positive and -negative sera. Signals were proven to be protein-specific by 
      inhibition experiments, and results agreed with Western blot experiments. The 
      potential of the assay to monitor IgG, IgM, and IgA responses over time was shown 
      in a proof-of-concept study with 116 sera from eight animals in a CBPP vaccine 
      study. In conclusion, a toolbox with recombinant proteins and a flexible 
      suspension array assay that allows multiplex analysis of humoral immune responses 
      to M. mycoides SC has been created.
FAU - Hamsten, Carl
AU  - Hamsten C
AD  - Department of Proteomics, School of Biotechnology, Royal Institute of Technology 
      (KTH), AlbaNova University Center, SE-106 91 Stockholm, Sweden.
FAU - Neiman, Maja
AU  - Neiman M
FAU - Schwenk, Jochen M
AU  - Schwenk JM
FAU - Hamsten, Marica
AU  - Hamsten M
FAU - March, John B
AU  - March JB
FAU - Persson, Anja
AU  - Persson A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090820
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Antibodies)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Proteome)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies/chemistry
MH  - Cattle
MH  - Escherichia coli/metabolism
MH  - *Immunity, Humoral
MH  - Immunoglobulin A/chemistry
MH  - Immunoglobulin G/chemistry
MH  - Immunoglobulin M/chemistry
MH  - Mycoplasma mycoides/*metabolism
MH  - Pleuropneumonia, Contagious/*microbiology
MH  - Protein Array Analysis
MH  - Proteome
MH  - Proteomics/methods
MH  - Recombinant Proteins/*chemistry
MH  - Surface Properties
MH  - Vaccines
PMC - PMC2773720
EDAT- 2009/08/22 09:00
MHDA- 2010/02/19 06:00
PMCR- 2010/11/01
CRDT- 2009/08/22 09:00
PHST- 2009/08/22 09:00 [entrez]
PHST- 2009/08/22 09:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
PHST- 2010/11/01 00:00 [pmc-release]
AID - S1535-9476(20)33939-6 [pii]
AID - M900009-MCP200 [pii]
AID - 10.1074/mcp.M900009-MCP200 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2009 Nov;8(11):2544-54. doi: 10.1074/mcp.M900009-MCP200. 
      Epub 2009 Aug 20.