PMID- 19697769
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20090824
IS  - 1004-5619 (Print)
IS  - 1004-5619 (Linking)
VI  - 25
IP  - 3
DP  - 2009 Jun
TI  - [Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion].
PG  - 161-3
AB  - OBJECTIVE: To investigate the expression of intercellular adhesion molecular-1 
      (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ 
      hybridization (FISH) and its relationship with the contusion interval. METHODS: 
      To make the contusion models with rats skeletal muscle. The samples were taken to 
      extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control 
      group after contusion respectively. FISH was performed on frozen section samples 
      and the sections were observed using LSCM. RESULTS: The expression of ICAM-1 mRNA 
      peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times 
      the level of control group at 18 h and then increased again. CONCLUSION: The 
      time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially 
      useful for estimation of early wound age.
FAU - Pei, Ming
AU  - Pei M
AD  - School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. 
      peiming5992@163.com
FAU - Sun, Jun-Hong
AU  - Sun JH
FAU - Gao, Cai-Rong
AU  - Gao CR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Fa Yi Xue Za Zhi
JT  - Fa yi xue za zhi
JID - 9426151
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Contusions/*metabolism/pathology
MH  - Female
MH  - Forensic Pathology
MH  - Hindlimb/*injuries
MH  - In Situ Hybridization, Fluorescence
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Male
MH  - Muscle, Skeletal/*injuries/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Wound Healing
EDAT- 2009/08/25 09:00
MHDA- 2010/02/03 06:00
CRDT- 2009/08/25 09:00
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
PST - ppublish
SO  - Fa Yi Xue Za Zhi. 2009 Jun;25(3):161-3.