PMID- 19698128
OWN - NLM
STAT- MEDLINE
DCOM- 20100113
LR  - 20211020
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 7
DP  - 2009 Aug 22
TI  - Modulation of cytokines and transcription factors (T-Bet and GATA3) in CD4 
      enriched cervical cells of Chlamydia trachomatis infected fertile and infertile 
      women upon stimulation with chlamydial inclusion membrane proteins B and C.
PG  - 84
LID - 10.1186/1477-7827-7-84 [doi]
AB  - BACKGROUND: Chlamydial Inclusion membrane proteins (Incs), are involved in 
      biochemical interactions with host cells and infecting Chlamydiae. We have 
      previously reported the role of two Chlamydia trachomatis (CT) Incs, namely IncB 
      and IncC in generating host immunity in CT infected women. Emerging data shows 
      involvement of Inc stimulated CD4 positive T cells in aiding host immunity in 
      infected fertile and infertile women through the secretion of interferon gamma. 
      However the lack of data on the intra-cytokine interplay to these Incs in 
      infected cell milieu prompted us to investigate further. METHODS: A total of 14 
      CT-positive fertile, 18 CT-positive infertile women and 25 uninfected controls 
      were enrolled in this study. CD8 depleted, CD4 enriched cervical cells were 
      isolated and upon stimulation with IncB and IncC, modulation of cytokines 
      (Interleukin (IL)-1 Beta, IL-4, IL-5, IL-6, IL-10, Interferon-gamma, IL-12, 
      IL-23, Tumor Necrosis Factor-alpha and Granulocyte macrophage colony-stimulating 
      factor (GM-CSF) and T cell lineage regulating transcription factors T-Bet and 
      GATA3 was determined by real-time reverse-transcriptase (RT)-PCR and ELISA. 
      RESULTS: Significant higher expression (P < 0.05) of Interferon-gamma, IL-12, 
      IL-23 and GM-CSF were found in Inc-stimulated CD4 enriched cervical cells of 
      CT-positive fertile women and contrastingly high IL-1 Beta, IL-4, IL-5, IL-6 and 
      IL-10 levels were found in CT-positive infertile women. Positive correlation (P < 
      0.05) was found between Interferon-gamma and T-Bet levels in CT-positive fertile 
      women and IL-4 mRNA and GATA3 levels in CT-positive infertile patients upon IncB 
      and IncC stimulation. CONCLUSION: Overall our data shows that CT IncB and IncC 
      are able to upregulate expression of cytokines, namely interferon-gamma, IL-12, 
      IL-23 and GM-CSF in CT-positive fertile women while expression of IL-1 Beta, 
      IL-4, IL-5, IL-6 and IL-10 were upregulated in CT-positive infertile women. Our 
      study also suggests that Incs are able to modulate expression of T cell lineage 
      determinants indicating their involvement in regulation of immune cells.
FAU - Gupta, Rishein
AU  - Gupta R
AD  - Institute of Pathology-ICMR, Safdarjang Hospital Campus, Post Box no, 4909, New 
      Delhi-110 029, India. rishein9@rediffmail.com
FAU - Vardhan, Harsh
AU  - Vardhan H
FAU - Srivastava, Pragya
AU  - Srivastava P
FAU - Salhan, Sudha
AU  - Salhan S
FAU - Mittal, Aruna
AU  - Mittal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090822
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-23)
RN  - 0 (Interleukin-5)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Proteins)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Bacterial Proteins/immunology
MH  - CD4-Positive T-Lymphocytes/immunology/*metabolism/virology
MH  - Cervix Uteri/cytology/immunology/virology
MH  - Chlamydia Infections/immunology/*metabolism/virology
MH  - Chlamydia trachomatis/immunology/physiology
MH  - Cytokines/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fertility/immunology
MH  - GATA3 Transcription Factor/genetics/*metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Infertility, Female/immunology
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-10/genetics/metabolism
MH  - Interleukin-12/genetics/metabolism
MH  - Interleukin-1beta/genetics/metabolism
MH  - Interleukin-23/genetics/metabolism
MH  - Interleukin-4/genetics/metabolism
MH  - Interleukin-5/genetics/metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - Membrane Proteins/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Box Domain Proteins/genetics/*metabolism
PMC - PMC2736965
EDAT- 2009/08/25 09:00
MHDA- 2010/01/14 06:00
PMCR- 2009/08/22
CRDT- 2009/08/25 09:00
PHST- 2009/05/27 00:00 [received]
PHST- 2009/08/22 00:00 [accepted]
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2010/01/14 06:00 [medline]
PHST- 2009/08/22 00:00 [pmc-release]
AID - 1477-7827-7-84 [pii]
AID - 10.1186/1477-7827-7-84 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2009 Aug 22;7:84. doi: 10.1186/1477-7827-7-84.