PMID- 1969886
OWN - NLM
STAT- MEDLINE
DCOM- 19900521
LR  - 20071114
IS  - 0737-1454 (Print)
IS  - 0737-1454 (Linking)
VI  - 8 Suppl 1
DP  - 1990 Jan
TI  - Human umbilical cord blood: a clinically useful source of transplantable 
      hematopoietic stem/progenitor cells.
PG  - 76-89; discussion 89-91
AB  - This is a review and discussion of studies leading to the first use of human 
      umbilical cord blood, material usually discarded, for the provision of 
      stem/progenitor cells for clinical hematopoietic reconstitution. This prospect 
      arose as a result of extensive studies of the harvesting and cryopreservation of 
      cord blood and of its numerical content of progenitor cells demonstrable in 
      vitro. A male patient with Fanconi anemia (FA) was conditioned with a modified 
      regimen of cyclophosphamide and irradiation that accommodates the abnormally high 
      sensitivity to these agents that is characteristic of FA. Cryopreserved cord 
      blood had been retrieved at birth from a female sibling known from prenatal 
      testing to be unaffected by FA and to be human leukocyte antigen (HLA)-compatible 
      with the prospective sibling recipient. After conditioning and therapeutic 
      infusion of thawed cord blood, successful hematopoietic reconstitution was 
      indicated by the general health of the patient, who had previously required 
      supportive transfusions, by satisfactory hematological criteria and by counts of 
      hematopoietic progenitor cells of various types in the bone marrow. Complete 
      engraftment of the myeloid system with donor cells was evident from cytogenetics, 
      ABO typing, study of DNA polymorphisms, and normal cellular resistance to 
      cytotoxic agents that reveal the fragility of FA cells; the blood contained a 
      residuum of host lymphocytes exhibiting chromosomal damage, but the trend has 
      been towards eliminating these damaged cells. This implies that cord blood from a 
      single individual should provide sufficient reconstituting cells for effective 
      hematopoietic repopulation of an autologous or an HLA-compatible allogeneic 
      recipient.
FAU - Broxmeyer, H E
AU  - Broxmeyer HE
AD  - Department of Medicine (Hematology/Oncology), Indiana University School of 
      Medicine, Indianapolis 46202-5121.
FAU - Gluckman, E
AU  - Gluckman E
FAU - Auerbach, A
AU  - Auerbach A
FAU - Douglas, G W
AU  - Douglas GW
FAU - Friedman, H
AU  - Friedman H
FAU - Cooper, S
AU  - Cooper S
FAU - Hangoc, G
AU  - Hangoc G
FAU - Kurtzberg, J
AU  - Kurtzberg J
FAU - Bard, J
AU  - Bard J
FAU - Boyse, E A
AU  - Boyse EA
LA  - eng
GR  - CA 36464/CA/NCI NIH HHS/United States
GR  - CA 36740/CA/NCI NIH HHS/United States
GR  - CA 39827/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - Int J Cell Cloning
JT  - International journal of cell cloning
JID - 8308172
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Blood Transfusion
MH  - Fanconi Anemia/immunology/therapy
MH  - *Fetal Blood/analysis
MH  - HLA Antigens/immunology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
RF  - 82
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1002/stem.5530080708 [doi]
PST - ppublish
SO  - Int J Cell Cloning. 1990 Jan;8 Suppl 1:76-89; discussion 89-91. doi: 
      10.1002/stem.5530080708.