PMID- 19699172
OWN - NLM
STAT- MEDLINE
DCOM- 20090925
LR  - 20211020
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 31
IP  - 2
DP  - 2009 Aug 21
TI  - A role for lipid bodies in the cross-presentation of phagocytosed antigens by MHC 
      class I in dendritic cells.
PG  - 232-44
LID - 10.1016/j.immuni.2009.06.022 [doi]
AB  - Dendritic cells (DCs) have the striking ability to cross-present exogenous 
      antigens in association with major histocompatibility complex (MHC) class I to 
      CD8(+) T cells. However, the intracellular pathways underlying cross-presentation 
      remain ill defined. Current models involve cytosolic proteolysis of antigens by 
      the proteasome and peptide import into endoplasmic reticulum (ER) or phagosomal 
      lumen by the transporters associated with antigen processing (TAP1 and TAP2). 
      Here, we show that DCs expressed an ER-resident 47 kDa immune-related GTPase, 
      Igtp (Irgm3). Igtp resides on ER and lipid body (LB) membranes where it binds the 
      LB coat component ADFP. Inactivation of genes encoding for either Igtp or ADFP 
      led to defects in LB formation in DCs and severely impaired cross-presentation of 
      phagocytosed antigens to CD8(+) T cells but not antigen presentation to CD4(+) T 
      cells. We thus define a new role for LB organelles in regulating 
      cross-presentation of exogenous antigens to CD8(+) T lymphocytes in DCs.
FAU - Bougneres, Laurence
AU  - Bougneres L
AD  - INSERM U653, Institut Curie, Section Recherche, 26, rue d'Ulm, 75248 Paris, Cedex 
      05, France.
FAU - Helft, Julie
AU  - Helft J
FAU - Tiwari, Sangeeta
AU  - Tiwari S
FAU - Vargas, Pablo
AU  - Vargas P
FAU - Chang, Benny Hung-Junn
AU  - Chang BH
FAU - Chan, Lawrence
AU  - Chan L
FAU - Campisi, Laura
AU  - Campisi L
FAU - Lauvau, Gregoire
AU  - Lauvau G
FAU - Hugues, Stephanie
AU  - Hugues S
FAU - Kumar, Pradeep
AU  - Kumar P
FAU - Kamphorst, Alice O
AU  - Kamphorst AO
FAU - Dumenil, Ana-Maria Lennon
AU  - Dumenil AM
FAU - Nussenzweig, Michel
AU  - Nussenzweig M
FAU - MacMicking, John D
AU  - MacMicking JD
FAU - Amigorena, Sebastian
AU  - Amigorena S
FAU - Guermonprez, Pierre
AU  - Guermonprez P
LA  - eng
GR  - R01 HL051586/HL/NHLBI NIH HHS/United States
GR  - R01 AI068041-01A1/AI/NIAID NIH HHS/United States
GR  - P01 AI051573-070004/AI/NIAID NIH HHS/United States
GR  - R01 AI068041/AI/NIAID NIH HHS/United States
GR  - P01 AI051573/AI/NIAID NIH HHS/United States
GR  - HL 51586/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lipids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Perilipin-2)
RN  - 0 (Plin2 protein, mouse)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (Igtp protein, mouse)
SB  - IM
CIN - Immunity. 2009 Aug 21;31(2):176-8. PMID: 19699167
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - *Cross-Priming
MH  - Dendritic Cells/*immunology
MH  - Endoplasmic Reticulum/immunology
MH  - GTP Phosphohydrolases/genetics/immunology/metabolism
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Lipids/*immunology
MH  - Membrane Proteins/genetics/immunology/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Perilipin-2
MH  - *Phagocytosis
PMC - PMC2803012
MID - NIHMS136509
COIS- The authors have no conflicting financial interests.
EDAT- 2009/08/25 09:00
MHDA- 2009/09/26 06:00
PMCR- 2010/08/21
CRDT- 2009/08/25 09:00
PHST- 2009/04/29 00:00 [received]
PHST- 2009/06/13 00:00 [revised]
PHST- 2009/06/18 00:00 [accepted]
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2009/09/26 06:00 [medline]
PHST- 2010/08/21 00:00 [pmc-release]
AID - S1074-7613(09)00325-2 [pii]
AID - 10.1016/j.immuni.2009.06.022 [doi]
PST - ppublish
SO  - Immunity. 2009 Aug 21;31(2):232-44. doi: 10.1016/j.immuni.2009.06.022.