PMID- 19699789
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20091019
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 126
IP  - 1
DP  - 2009 Oct 29
TI  - The inhibitory effect of Gynostemma pentaphyllum on MCP-1 and type I procollagen 
      expression in rat hepatic stellate cells.
PG  - 42-9
LID - 10.1016/j.jep.2009.08.012 [doi]
AB  - AIM OF THE STUDY: Gynostemma pentaphyllum is a popular folk medicine that has 
      been used for treatment of hepatitis in Asia. Our previous study demonstrates 
      that Gynostemma pentaphyllum n-butanol extract inhibits the onset and improves 
      the recovery of CCl(4)-induced liver fibrogenesis in rats and inhibits 
      PDGF-induced rat hepatic stellate cells (HSCs) proliferation. In this study, the 
      effect of Gynostemma pentaphyllum extract on cytokines and type I procollagen 
      expression was analyzed. MATERIALS AND METHODS: Rat HSCs were treated with PDGF, 
      Gynostemma pentaphyllum n-butanol extract, RP-18-Gyp fraction, rapamycin or 
      vehicle. Rat cytokine antibody array chip or ELISA kit was used for cytokines 
      detection. Intracellular protein expression was detected by Western blotting, 
      mRNA expression was analyzed by RT-PCR. RESULTS: RP-18-Gyp fraction is the more 
      purified gypenosides fraction from Gynostemma pentaphyllum n-butanol extract. In 
      cell proliferation, the inhibitory effect of 200 microg/ml RP-18-Gyp fraction is 
      similar to 500 microg/ml Gynostemma pentaphyllum n-butanol extract. Furthermore, 
      both of them have the ability of decreasing monocyte chemoattractant protein-1 
      (MCP-1) mRNA expression and protein release and inhibiting type I procollagen 
      protein expression. CONCLUSIONS: Both of Gynostemma pentaphyllum n-butanol 
      extract and its more purified RP-18-Gyp fraction have the biological activities 
      in the inhibition of cell proliferation, MCP-1 release and type I procollagen 
      expression in rat HSCs. These data could provide the evidence to support for the 
      traditional use of Gynostemma pentaphyllum in hepatitis.
FAU - Chen, Ming-Ho
AU  - Chen MH
AD  - Department of Chinese Medicine, Chiayi Christian Hospital, Chiayi, Taiwan.
FAU - Wang, Qwa-Fun
AU  - Wang QF
FAU - Chen, Lih-Geeng
AU  - Chen LG
FAU - Shee, Jia-Jen
AU  - Shee JJ
FAU - Chen, Jung-Chou
AU  - Chen JC
FAU - Chen, Ke-Yu
AU  - Chen KY
FAU - Chen, Shu-Hsin
AU  - Chen SH
FAU - Su, Jyan-Gwo J
AU  - Su JG
FAU - Liu, Yi-Wen
AU  - Liu YW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090821
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Collagen Type I)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (gypenoside)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/*metabolism
MH  - Collagen Type I/*metabolism
MH  - Cytokines/metabolism
MH  - Drugs, Chinese Herbal/pharmacology
MH  - Gene Expression/drug effects
MH  - Gynostemma/chemistry
MH  - Hepatic Stellate Cells/*drug effects/metabolism
MH  - Male
MH  - Plant Extracts/chemistry/pharmacology
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2009/08/25 09:00
MHDA- 2010/02/18 06:00
CRDT- 2009/08/25 09:00
PHST- 2008/09/09 00:00 [received]
PHST- 2009/08/11 00:00 [revised]
PHST- 2009/08/12 00:00 [accepted]
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - S0378-8741(09)00507-8 [pii]
AID - 10.1016/j.jep.2009.08.012 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2009 Oct 29;126(1):42-9. doi: 10.1016/j.jep.2009.08.012. Epub 
      2009 Aug 21.