PMID- 19700193
OWN - NLM
STAT- MEDLINE
DCOM- 20091020
LR  - 20101118
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 46
IP  - 16
DP  - 2009 Oct
TI  - Suboptimal recognition of a T cell epitope of the major dog allergen Can f 1 by 
      human T cells.
PG  - 3320-7
LID - 10.1016/j.molimm.2009.07.022 [doi]
AB  - We have previously proposed that mammalian lipocalin allergens are recognized 
      suboptimally by the human immune system due to their homology with endogenous 
      lipocalins. Here, we have characterized in detail the human T cell recognition of 
      one of the previously identified T cell epitopes of the major dog allergen Can f 
      1, contained in peptide p105-120. A panel of peptide analogues (altered peptide 
      ligands, APLs) of p105-120 was tested on two specific T cell clones restricted by 
      different human leukocyte antigen (HLA) alleles. Interestingly, we identified for 
      both of the clones several heteroclitic APLs that were capable of stimulating 
      them at 10-30-fold lower concentrations than the natural peptide. Moreover, one 
      of the heteroclitic APLs identified with the T cell clones, L115F, was observed 
      to induce a stronger polyclonal T cell response than the natural allergen peptide 
      from the peripheral blood mononuclear cells (PBMCs) of six Can f 1-allergic 
      subjects studied. The heteroclitic APLs bound with the same affinity as p105-120 
      to common HLA-DR- and HLA-DP-alleles, suggesting that their improved stimulatory 
      capacity is attributable to a more efficient T cell receptor (TCR) recognition 
      rather than increased HLA binding. Collectively, our data suggest that p105-120 
      is recognized suboptimally by human T cells. This may contribute to the 
      allergenicity of Can f 1.
FAU - Juntunen, Riikka
AU  - Juntunen R
AD  - Department of Clinical Microbiology, Institute of Clinical Medicine and Biocenter 
      Kuopio, University of Kuopio, Kuopio, Finland.
FAU - Liukko, Aino
AU  - Liukko A
FAU - Taivainen, Antti
AU  - Taivainen A
FAU - Narvanen, Ale
AU  - Narvanen A
FAU - Durand, Guillaume
AU  - Durand G
FAU - Kauppinen, Anu
AU  - Kauppinen A
FAU - Nieminen, Anssi
AU  - Nieminen A
FAU - Rytkonen-Nissinen, Marja
AU  - Rytkonen-Nissinen M
FAU - Saarelainen, Soili
AU  - Saarelainen S
FAU - Maillere, Bernard
AU  - Maillere B
FAU - Virtanen, Tuomas
AU  - Virtanen T
FAU - Kinnunen, Tuure
AU  - Kinnunen T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090822
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipocalins)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (allergen Can f I)
SB  - IM
MH  - Alleles
MH  - Allergens/genetics/*immunology
MH  - Animals
MH  - Antigens, Plant
MH  - Cells, Cultured
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Epitopes, T-Lymphocyte/genetics/*immunology
MH  - HLA-DP Antigens/genetics/immunology
MH  - HLA-DR Antigens/genetics/immunology
MH  - Humans
MH  - Hypersensitivity/genetics/*immunology
MH  - Lipocalins/genetics/immunology
MH  - Peptides/genetics/*immunology/pharmacology
MH  - Receptors, Antigen, T-Cell/genetics/*immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2009/08/25 09:00
MHDA- 2009/10/21 06:00
CRDT- 2009/08/25 09:00
PHST- 2009/06/25 00:00 [received]
PHST- 2009/07/26 00:00 [accepted]
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2009/10/21 06:00 [medline]
AID - S0161-5890(09)00623-3 [pii]
AID - 10.1016/j.molimm.2009.07.022 [doi]
PST - ppublish
SO  - Mol Immunol. 2009 Oct;46(16):3320-7. doi: 10.1016/j.molimm.2009.07.022. Epub 2009 
      Aug 22.