PMID- 19701586
OWN - NLM
STAT- MEDLINE
DCOM- 20150421
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 61
IP  - 4
DP  - 2009 Aug 25
TI  - Adrenomedullin in the rostral ventrolateral medulla is involved in the regulation 
      of cardiovascular component of defensive responses induced by electrical 
      stimulation of dorsal periaquaductal gray of the midbrain.
PG  - 347-55
AB  - In this study, we used techniques of in situ hybridization, immunohistochemistry, 
      electric stimulation of the dorsal periaquaductal gray of the midbrain (dPAG) and 
      microinjection to investigate the changes of preproadrenomedullin (ppADM) gene 
      expression encoding adrenomedullin (ADM) and ADM-like immunoreactivity (ADM-IR) 
      in the medulla oblongata, especially in the rostral ventrolateral medulla (rVLM) 
      of the rats receiving foot-shock and noise stress for 5 d, and the potential role 
      of ADM in cardiovascular component of defense response in the rVLM. The results 
      showed that ppADM mRNA and ADM-IR were widely distributed throughout the medulla 
      oblongata. Highly labeled neurons were found in the ventrolateral reticular 
      nucleus and hypoglossal nucleus. Moderately labeled neurons were seen in the 
      facial, ambiguus, lateral reticular, paragigantocellular reticular, and inferior 
      olivary nuclei. Weak signal was present over neurons of nucleus of the solitary 
      tract. The expression of ppADM mRNA and ADM-IR increased significantly after foot 
      shock and noise stress for 5 d as compared with that in control group (P<0.01). 
      On the other hand, stimulation of the right dPAG raised the artery pressure (AP) 
      rapidly from (116.4+/-8.9) mmHg to (140.0+/-9.8) mmHg, and heart rate (HR) from 
      (378.0+/-7.5) beats/min to (413.0+/-8.2) beats/min, respectively, in the 
      normotensive rats. After unilaterally microinjection of hADM(22-52) (a specific 
      antagonist of ADM receptor, 1 pmol) into the right rVLM of the normotensive rats 
      for 10 min, the rats received the stimulation of the dPAG again. Then we found 
      that the DeltaAP and DeltaHR were lowered significantly within 60 min compared 
      with those without hADM(22-52) application (P<0.05). After unilaterally 
      microinjection of 0.1 pmol rat ADM (rADM) into the rVLM, dPAG stimulation caused 
      no significant changes in DeltaAP and DeltaHR. Our results that foot-shock and 
      noise stress induced significant increases of ppADM mRNA and ADM-IR in the rVLM, 
      and microinjection of ADM receptor antagonist hADM(22-52) into the rVLM partly 
      blocked the cardiovascular component of stress-defensive response induced by 
      stimulation of the dPAG, suggest that ADM in the rVLM might be an important 
      neurotransmitter or neuroregulator in the regulation of cardiovascular function 
      in the stress-related defensive response.
FAU - Li, Xia
AU  - Li X
AD  - Department of Internal Neurology, Huashan Hospital Affiliated to Fudan 
      University, Shanghai 200040, China.
FAU - Fan, Ming-Xin
AU  - Fan MX
FAU - Li, Liang
AU  - Li L
FAU - Wang, Jin
AU  - Wang J
FAU - Shen, Lin-Lin
AU  - Shen LL
FAU - Cao, Yin-Xiang
AU  - Cao YX
FAU - Zhu, Da-Nian
AU  - Zhu DN
FAU - Hong, Zhen
AU  - Hong Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Protein Precursors)
RN  - 0 (preproadrenomedullin)
RN  - 148498-78-6 (Adrenomedullin)
SB  - IM
MH  - Adrenomedullin/*physiology
MH  - Animals
MH  - Blood Pressure
MH  - Electric Stimulation
MH  - Heart Rate
MH  - Medulla Oblongata/*physiology
MH  - Microinjections
MH  - Neurons/physiology
MH  - Periaqueductal Gray/*physiology
MH  - Protein Precursors/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2009/08/25 09:00
MHDA- 2015/04/22 06:00
CRDT- 2009/08/25 09:00
PHST- 2009/08/25 09:00 [entrez]
PHST- 2009/08/25 09:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2009 Aug 25;61(4):347-55.