PMID- 19702848
OWN - NLM
STAT- MEDLINE
DCOM- 20091015
LR  - 20090825
IS  - 1523-5378 (Electronic)
IS  - 1083-4389 (Linking)
VI  - 14
IP  - 3
DP  - 2009 Jun
TI  - Spatial distribution of Helicobacter spp. in the gastrointestinal tract of dogs.
PG  - 180-91
LID - 10.1111/j.1523-5378.2009.00674.x [doi]
AB  - BACKGROUND: In dogs, the gastric Helicobacter spp. have been well studied, but 
      there is little information regarding the other parts of the alimentary system. 
      We sought to determine the spatial distribution of Helicobacter spp. in the 
      gastrointestinal tract and the hepatobiliary system of dogs using 
      culture-independent methods. MATERIALS AND METHODS: Samples of stomach, duodenum, 
      ileum, cecum, colon, pancreas, liver, and bile from six dogs were evaluated for 
      Helicobacter spp. by genus, gastric, and enterohepatic Helicobacter spp. 
      Polymerase chain reaction, 16S rRNA gene sequence analysis, immunohistochemistry, 
      and fluorescence in situ hybridization (FISH). RESULTS: In the stomach, 
      Helicobacter spp. DNA was detected in all six dogs, with H. bizzozeronii and H. 
      felis identified by specific polymerase chain reaction. Helicobacter organisms 
      were localized within the surface mucus, the lumen of gastric glands, and inside 
      parietal cells. The small intestine harbored gastric and enterohepatic 
      Helicobacter spp. DNA/antigen in low amounts. In the cecum and colon, 
      Helicobacter spp. DNA, with highest similarity to H. bilis/flexispira taxon 8, H. 
      cinaedi, and H. canis, was detected in all six dogs. Helicobacter organisms were 
      localized at the mucosal surface and within the crypts. Gastric Helicobacter spp. 
      DNA was detected occasionally in the large intestine, but no gastric Helicobacter 
      spp. were present in clone libraries or detected by FISH. CONCLUSIONS: This study 
      demonstrates that in addition to the stomach, the large intestine of dogs is also 
      abundantly colonized by Helicobacter spp. Additional studies are necessary to 
      investigate the association between enterohepatic Helicobacter spp. and presence 
      of intestinal inflammatory or proliferative disorders in dogs.
FAU - Recordati, Camilla
AU  - Recordati C
AD  - Dipartimento di Patologia Animale, Igiene e Sanita Pubblica Veterinaria, Sezione 
      di Anatomia Patologica Veterinaria e Patologia Aviare, Facolta di Medicina 
      Veterinaria, Universita degli Studi di Milano, Milano, Italy. 
      camilla.recordati@unimi.it
FAU - Gualdi, Valentina
AU  - Gualdi V
FAU - Craven, Melanie
AU  - Craven M
FAU - Sala, Lorenza
AU  - Sala L
FAU - Luini, Mario
AU  - Luini M
FAU - Lanzoni, Anna
AU  - Lanzoni A
FAU - Rishniw, Mark
AU  - Rishniw M
FAU - Simpson, Kenneth W
AU  - Simpson KW
FAU - Scanziani, Eugenio
AU  - Scanziani E
LA  - eng
PT  - Journal Article
PL  - England
TA  - Helicobacter
JT  - Helicobacter
JID - 9605411
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Dog Diseases/*microbiology
MH  - Dogs
MH  - Female
MH  - Gastrointestinal Tract/*microbiology
MH  - Helicobacter/genetics/*isolation & purification
MH  - Helicobacter Infections/microbiology/*veterinary
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2009/08/26 09:00
MHDA- 2009/10/16 06:00
CRDT- 2009/08/26 09:00
PHST- 2009/08/26 09:00 [entrez]
PHST- 2009/08/26 09:00 [pubmed]
PHST- 2009/10/16 06:00 [medline]
AID - HEL674 [pii]
AID - 10.1111/j.1523-5378.2009.00674.x [doi]
PST - ppublish
SO  - Helicobacter. 2009 Jun;14(3):180-91. doi: 10.1111/j.1523-5378.2009.00674.x.