PMID- 19702874
OWN - NLM
STAT- MEDLINE
DCOM- 20091127
LR  - 20171116
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 70
IP  - 2
DP  - 2009 Nov
TI  - Influence of intercropping and intercropping plus rhizobial inoculation on 
      microbial activity and community composition in rhizosphere of alfalfa (Medicago 
      sativa L.) and Siberian wild rye (Elymus sibiricus L.).
PG  - 62-70
LID - 10.1111/j.1574-6941.2009.00752.x [doi]
AB  - Alfalfa-Siberian wild rye intercropping is the predominant cropping system used 
      to produce forage in China. In this study, the effects of intercropping and 
      intercropping-rhizobial inoculation on soil enzyme activities, microbial biomass 
      and bacterial community composition in the rhizosphere were examined. In both 
      treatments, the yield of alfalfa, microbial biomass and activities of soil 
      urease, invertase and alkaline phosphatase in the alfalfa rhizosphere were 
      markedly increased, whereas there was a slight increase in the yield of Siberian 
      wild rye, few impacts on soil microbial biomass, and decreased enzyme activities 
      (except for urease) in the Siberian wild rye rhizosphere. Terminal restriction 
      fragment length polymorphism (T-RFLP) of 16S rRNA genes indicated that 
      Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, 
      Deltaproteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were the major 
      bacterial groups in the rhizosphere of both plants. However, intercropping and 
      rhizobial inoculation induced some shifts in the relative abundance of them. 
      Nitrosomonas and Nitrosospira groups were detected in all treatments by the 
      T-RFLP patterns of ammonia monooxygenase subunit A (amoA) gene, but the relative 
      abundance of Nitrosomonas increased and that of Nitrosospira decreased in the 
      intercropping-rhizobial inoculation treatment. Both treatments tended to increase 
      the diversity of amoA. Conclusively, the two treatments clearly affected soil 
      microbial composition and soil enzyme activities, which might be reflected in 
      changes in yield.
FAU - Sun, Yan Mei
AU  - Sun YM
AD  - State Key Laboratory for Agrobiotechnology, College of Biological Sciences and 
      Center for Biomass Engineering, China Agricultural University, Beijing, China.
FAU - Zhang, Nan Nan
AU  - Zhang NN
FAU - Wang, En Tao
AU  - Wang ET
FAU - Yuan, Hong Li
AU  - Yuan HL
FAU - Yang, Jin Shui
AU  - Yang JS
FAU - Chen, Wen Xin
AU  - Chen WX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090729
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
SB  - IM
MH  - Agriculture/*methods
MH  - Bacteria/classification/enzymology/*genetics/isolation & purification
MH  - Biodiversity
MH  - Biomass
MH  - DNA, Bacterial/genetics
MH  - Elymus/*microbiology
MH  - Medicago sativa/*microbiology
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Soil/analysis
MH  - *Soil Microbiology
EDAT- 2009/08/26 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/08/26 09:00
PHST- 2009/08/26 09:00 [entrez]
PHST- 2009/08/26 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - FEM752 [pii]
AID - 10.1111/j.1574-6941.2009.00752.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2009 Nov;70(2):62-70. doi: 10.1111/j.1574-6941.2009.00752.x. 
      Epub 2009 Jul 29.