PMID- 19703762
OWN - NLM
STAT- MEDLINE
DCOM- 20100304
LR  - 20151119
IS  - 1532-3064 (Electronic)
IS  - 0954-6111 (Linking)
VI  - 103
IP  - 12
DP  - 2009 Dec
TI  - Potential diagnostic biomarkers in serum of idiopathic pulmonary arterial 
      hypertension.
PG  - 1801-6
LID - 10.1016/j.rmed.2009.07.017 [doi]
AB  - BACKGROUND: The pathogenesis of idiopathic pulmonary arterial hypertension (IPAH) 
      is unknown, and the syndrome of IPAH remains a diagnostic and therapeutic 
      challenge. The present study investigated the disease-specific proteins that aid 
      in the diagnosis of IPAH and thus to study their role in the disease process. 
      METHODS: A comparative proteomic analysis was used for clinical screening of 
      serum proteins in 10 patients with IPAH and compared with 10 normal subjects. 
      Furthermore, enzyme linked immunosorbent assay (ELISA) was performed for 
      comparison with serum proteins between individual IPAH patients and controls. 
      RESULTS: Nine proteins and their isoforms, including leucine-rich 
      alpha-2-glycoprotein (LRG), haptoglobin precursor, albumin isoform 2, transferrin 
      variant, C3 complement, hydroxypyruvate reductase isoform 1, RAF1, fibrinogen 
      isoformgamma-A and fibrinogen isoformgamma-B showed significant changes in serum 
      of IPAH patients compared with controls by proteomic analysis. And significant 
      higher serum levels of LRG in IPAH patients compared with controls were found by 
      ELISA. Correlation analysis disclosed a significant association between serum LRG 
      concentrations and New York Heart Association (NYHA) functional class (r=0.71, 
      P<0.01) and cardiac output (CO) (r=-0.65, P<0.01). CONCLUSIONS: These results 
      indicate that there are significant differences in the expression of proteins in 
      the serum of patients with IPAH and normal subjects. And the measurement of LRG, 
      RAF1 and C3 complement levels in the serum may be helpful for the diagnosis of 
      IPAH. In particular, LRG may be a specific prognostical biomarker of IPAH.
FAU - Zhang, Jianqiang
AU  - Zhang J
AD  - Research Center for Pathology and Physiology, Cardiovascular Institute and Fu Wai 
      Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 
      167 Bei-Li-Shi Road, Xi Cheng District, Beijing 100037, China.
FAU - Zhang, Ying
AU  - Zhang Y
FAU - Li, Ning
AU  - Li N
FAU - Liu, Zhihong
AU  - Liu Z
FAU - Xiong, Changming
AU  - Xiong C
FAU - Ni, Xinhai
AU  - Ni X
FAU - Pu, Yaoli
AU  - Pu Y
FAU - Hui, Rutai
AU  - Hui R
FAU - He, Jianguo
AU  - He J
FAU - Pu, Jielin
AU  - Pu J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20090822
PL  - England
TA  - Respir Med
JT  - Respiratory medicine
JID - 8908438
RN  - 0 (Biomarkers)
RN  - 0 (Glycoproteins)
RN  - 0 (Proteins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glycoproteins/blood
MH  - Humans
MH  - Hypertension, Pulmonary/blood/*diagnosis
MH  - Male
MH  - Proteins/*analysis
MH  - Proto-Oncogene Proteins c-raf/blood
MH  - Young Adult
EDAT- 2009/08/26 09:00
MHDA- 2010/03/05 06:00
CRDT- 2009/08/26 09:00
PHST- 2009/04/20 00:00 [received]
PHST- 2009/07/02 00:00 [revised]
PHST- 2009/07/26 00:00 [accepted]
PHST- 2009/08/26 09:00 [entrez]
PHST- 2009/08/26 09:00 [pubmed]
PHST- 2010/03/05 06:00 [medline]
AID - S0954-6111(09)00249-2 [pii]
AID - 10.1016/j.rmed.2009.07.017 [doi]
PST - ppublish
SO  - Respir Med. 2009 Dec;103(12):1801-6. doi: 10.1016/j.rmed.2009.07.017. Epub 2009 
      Aug 22.