PMID- 19707549
OWN - NLM
STAT- MEDLINE
DCOM- 20100119
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 8
DP  - 2009 Aug 26
TI  - Identification of genes responsive to solar simulated UV radiation in human 
      monocyte-derived dendritic cells.
PG  - e6735
LID - 10.1371/journal.pone.0006735 [doi]
LID - e6735
AB  - Ultraviolet (UV) irradiation has profound effects on the skin and the systemic 
      immune system. Several effects of UV radiation on Dendritic cells (DCs) functions 
      have been described. However, gene expression changes induced by UV radiation in 
      DCs have not been addressed before. In this report, we irradiated human 
      monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on 
      human whole genome arrays. Results were validated by RT-PCR and further analyzed 
      by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated 
      expression of genes involved in cellular stress and inflammation, and 
      down-regulated genes involved in chemotaxis, vesicular transport and RNA 
      processing. Twenty four genes were selected for comparison by RT-PCR with 
      similarly treated human primary keratinocytes and human melanocytes. Several 
      genes involved in the regulation of the immune response were differentially 
      regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein 
      tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), 
      inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), 
      IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation 
      of a complex gene repertoire involved in human-DC-mediated immune responses.
FAU - de la Fuente, Hortensia
AU  - de la Fuente H
AD  - Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 
      Madrid, Spain.
FAU - Lamana, Amalia
AU  - Lamana A
FAU - Mittelbrunn, Maria
AU  - Mittelbrunn M
FAU - Perez-Gala, Silvia
AU  - Perez-Gala S
FAU - Gonzalez, Salvador
AU  - Gonzalez S
FAU - Garcia-Diez, Amaro
AU  - Garcia-Diez A
FAU - Vega, Miguel
AU  - Vega M
FAU - Sanchez-Madrid, Francisco
AU  - Sanchez-Madrid F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090826
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism/*radiation effects
MH  - Down-Regulation/drug effects
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*radiation effects
MH  - Humans
MH  - Monocytes/metabolism/*radiation effects
MH  - *Ultraviolet Rays
MH  - Up-Regulation/drug effects
PMC - PMC2727914
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/08/27 09:00
MHDA- 2010/01/20 06:00
PMCR- 2009/08/26
CRDT- 2009/08/27 09:00
PHST- 2009/05/29 00:00 [received]
PHST- 2009/07/27 00:00 [accepted]
PHST- 2009/08/27 09:00 [entrez]
PHST- 2009/08/27 09:00 [pubmed]
PHST- 2010/01/20 06:00 [medline]
PHST- 2009/08/26 00:00 [pmc-release]
AID - 09-PONE-RA-10675R1 [pii]
AID - 10.1371/journal.pone.0006735 [doi]
PST - epublish
SO  - PLoS One. 2009 Aug 26;4(8):e6735. doi: 10.1371/journal.pone.0006735.