PMID- 19709242
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20090827
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 41
IP  - 2
DP  - 2002 Aug 1
TI  - Molecular characterization of microbial community in nitrate-removing activated 
      sludge.
PG  - 85-94
LID - 10.1111/j.1574-6941.2002.tb00969.x [doi]
AB  - Abstract The microbial community composition and dominant denitrifying 
      populations in high-nitrate-removing (CR-I) and low-nitrate-removing (CR-II) 
      activated sludge from continuous bioreactors were investigated with most probable 
      number (MPN) enumeration, fluorescence in situ hybridization (FISH) and 16S rDNA 
      characterization. MPNs of nitrate-reducing bacteria of sludge CR-I and sludge 
      CR-II were 2.82x10(7) and 2.69x10(4) colony-forming units ml(-1), respectively. 
      Eight denitrifying bacteria and two nitrate-reducing bacteria were isolated from 
      sludge CR-I, and four denitrifying bacteria and three nitrate-reducing bacteria 
      from sludge CR-II. Small subunit rDNA characterization of the isolates showed 
      that the majority belonged to the genus Pseudomonas. By using FISH up to 76% 
      (CR-I) and 52% (CR-II) of total 4,6-diamidino-2-phenylindole cell counts 
      hybridized to the bacterial probe EUB338. Members of beta-Proteobacteria were the 
      most abundant proteobacterial group in both sludges, accounting for up to 41.6% 
      and 37.1% of those detected by EUB338, respectively, whereas a higher number of 
      Cytophaga-Flexibacter cluster members were observed in CR-I sludge compared to 
      CR-II sludge. In contrast with culture-based results, the numbers of rRNA group I 
      Pseudomonads accounted for less than 0.01% of those detected by EUB338 in both 
      sludges. Ribosomal DNA clone library analysis showed that the beta-Proteobacteria 
      were also dominant in both sludges. In CR-I sludge, they were related to 
      Zooglorea ramigera, Alcaligenes defragrans, denitrifying Fe-oxidizing bacteria 
      and Dechlorimonas sp., whereas in CR-II sludge, they were related to Nitrosomonas 
      sp. and Dechlorimonas agitatus. When this reactor was operated under anaerobic 
      and anoxic conditions, nitrifying bacteria could adapt to the anoxic environment. 
      We inferred that anaerobic ammonium oxidation and nitrite oxidation may occur in 
      low-nitrate-removing sludge CR-II and inhibit denitrification.
FAU - Lee, Han-Woong
AU  - Lee HW
AD  - Graduate School of Biotechnology, Korea University, 1, 5 ka Anam-dong, 
      Sungbuk-ku, Seoul 136-701, South Korea.
FAU - Lee, Soo-Youn
AU  - Lee SY
FAU - Lee, Jin-Woo
AU  - Lee JW
FAU - Park, Jong-Bok
AU  - Park JB
FAU - Choi, Eui-So
AU  - Choi ES
FAU - Park, Yong Keun
AU  - Park YK
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
EDAT- 2002/08/01 00:00
MHDA- 2002/08/01 00:01
CRDT- 2009/08/28 09:00
PHST- 2009/08/28 09:00 [entrez]
PHST- 2002/08/01 00:00 [pubmed]
PHST- 2002/08/01 00:01 [medline]
AID - FEM85 [pii]
AID - 10.1111/j.1574-6941.2002.tb00969.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2002 Aug 1;41(2):85-94. doi: 
      10.1111/j.1574-6941.2002.tb00969.x.