PMID- 19709270
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20090827
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 42
IP  - 1
DP  - 2002 Oct 1
TI  - Sulfate-reducing bacterial community response to carbon source amendments in 
      contaminated aquifer microcosms.
PG  - 109-18
LID - 10.1111/j.1574-6941.2002.tb01000.x [doi]
AB  - Abstract Microbial sulfate reduction is an important metabolic activity in many 
      reduced habitats. However, little is known about the sulfate-reducing communities 
      inhabiting petroleum hydrocarbon (PHC)-contaminated freshwater aquifer sediments. 
      The purpose of this study was to identify the groups of sulfate-reducing bacteria 
      (SRB) selectively stimulated when sediment from a PHC-contaminated freshwater 
      aquifer was incubated in sulfate-reducing aquifer microcosms that were amended 
      with specific carbon sources (acetate, butyrate, propionate, lactate, and 
      citrate). After 2 months of incubation, the SRB community was characterized using 
      phospholipid fatty acid (PLFA) analysis combined with multivariate statistics as 
      well as fluorescence in situ hybridization (FISH). Molybdate was used to 
      specifically inhibit SRB in separate microcosms to investigate the contribution 
      of non-SRB to carbon source degradation. Results indicated that sulfate reduction 
      in the original sediment was an important process but was limited by the 
      availability of sulfate. Substantially lower amounts of acetate and butyrate were 
      degraded in molybdate treatments as compared to treatments without molybdate, 
      suggesting that SRB were the major bacterial group responsible for carbon source 
      turnover in microcosms. All of the added carbon sources induced changes in the 
      SRB community structure. Members of the genus Desulfobulbus were present but not 
      active in the original sediment but an increase of the fatty acids 15:1omega6c 
      and 17:1omega6c and FISH results showed an enrichment of these bacteria in 
      microcosms amended with propionate or lactate. The appearance of cy17:0 revealed 
      that bacteria affiliated with the Desulfobacteriaceae were responsible for 
      acetate degradation. Desulfovibrio and Desulfotomaculum spp. were not important 
      populations within the SRB community in microcosms because they did not 
      proliferate on carbon sources usually favored by these organisms. Metabolic, 
      PLFA, and FISH results provided information on the SRB community in a 
      PHC-contaminated freshwater environment, which exhibited stimulation patterns 
      similar to other (e.g. marine) environments.
FAU - Kleikemper, Jutta
AU  - Kleikemper J
AD  - Institute of Terrestrial Ecology, Swiss Federal Institute of Technology Zurich 
      (ETHZ), CH-8952 Schlieren, Switzerland.
FAU - Pelz, Oliver
AU  - Pelz O
FAU - Schroth, Martin H
AU  - Schroth MH
FAU - Zeyer, Josef
AU  - Zeyer J
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
EDAT- 2002/10/01 00:00
MHDA- 2002/10/01 00:01
CRDT- 2009/08/28 09:00
PHST- 2009/08/28 09:00 [entrez]
PHST- 2002/10/01 00:00 [pubmed]
PHST- 2002/10/01 00:01 [medline]
AID - FEM109 [pii]
AID - 10.1111/j.1574-6941.2002.tb01000.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2002 Oct 1;42(1):109-18. doi: 
      10.1111/j.1574-6941.2002.tb01000.x.