PMID- 19709757
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20141120
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 133
IP  - 2-4
DP  - 2010 Feb 15
TI  - Induction of inducible CD4+CD25+Foxp3+ regulatory T lymphocytes by porcine 
      reproductive and respiratory syndrome virus (PRRSV).
PG  - 170-82
LID - 10.1016/j.vetimm.2009.07.012 [doi]
AB  - Increases in numbers or activities of regulatory T lymphocytes (Tregs) have been 
      linked to the establishments of several persistent infections. It has been 
      previously shown that porcine reproductive and respiratory syndrome virus (PRRSV) 
      can negatively modulate the host immune responses, resulting in persistent 
      infection and secondary immunodeficiency. Recently, the existence of porcine 
      CD4(+)CD25(+) Tregs has been demonstrated. We investigated the effect of PRRSV on 
      the CD4(+)CD25(+) Tregs. The CD4(+)CD25(+)Foxp3(+) T lymphocytes in the 
      peripheral blood mononuclear cells (PBMCs) were identified, using the anti-human 
      anti-Foxp3 monoclonal antibody. In vitro culture of porcine PBMC in the presence 
      of PRRSV, but not classical swine fever virus, significantly increased the 
      numbers of Foxp3(+) lymphocytes, particularly in the CD4(+)CD25(high) 
      subpopulation. The time-course study revealed that PRRSV significantly increased 
      the numbers of viral-specific CD4(+)CD25(high)Foxp3(+) subpopulation in the 
      culture starting from 12h through the end of the observation period. Consistent 
      to the results obtained by flow cytometry, enhanced Foxp3 gene expression was 
      observed in the PBMC cultured with PRRSV in a time-course manner. The presence of 
      monocyte-derived DC in the co-culture significantly enhanced the induction of 
      CD4(+)CD25(+) Foxp3(+) T lymphocytes. The PRRSV-induced CD4(+)CD25(high) T 
      lymphocytes exhibited suppressive activity when co-cultured with PHA-activated, 
      autologous peripheral blood leukocytes, indicating the suppressive activity of 
      the PRRSV-specific Tregs. In addition, PRRSV exposure significantly increased the 
      numbers of PRRSV-specific CD4(+)CD25(+)Foxp3(+) subpopulation in the PBMC of 
      infected pigs at 10 days post-infection. In summary, the results indicated that 
      PRRSV could increase the numbers of viral-specific, inducible regulatory T 
      lymphocytes in the porcine PBMC, both in vitro and in vivo. The findings 
      suggested the novel immunomodulatory mechanism induced by PRRSV.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Wongyanin, P
AU  - Wongyanin P
AD  - Interdisciplinary Program of Medical Microbiology, Graduate School, Chulalongkorn 
      University, Bangkok 10330, Thailand.
FAU - Buranapraditkun, S
AU  - Buranapraditkun S
FAU - Chokeshai-Usaha, K
AU  - Chokeshai-Usaha K
FAU - Thanawonguwech, R
AU  - Thanawonguwech R
FAU - Suradhat, S
AU  - Suradhat S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090803
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/immunology
MH  - Forkhead Transcription Factors/genetics/metabolism
MH  - Gene Expression
MH  - In Vitro Techniques
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Lymphocyte Activation
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - Sus scrofa/genetics/*immunology/*virology
MH  - Swine
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Time Factors
EDAT- 2009/08/28 09:00
MHDA- 2010/04/17 06:00
CRDT- 2009/08/28 09:00
PHST- 2008/11/06 00:00 [received]
PHST- 2009/07/01 00:00 [revised]
PHST- 2009/07/28 00:00 [accepted]
PHST- 2009/08/28 09:00 [entrez]
PHST- 2009/08/28 09:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - S0165-2427(09)00244-X [pii]
AID - 10.1016/j.vetimm.2009.07.012 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):170-82. doi: 
      10.1016/j.vetimm.2009.07.012. Epub 2009 Aug 3.