PMID- 19713464
OWN - NLM
STAT- MEDLINE
DCOM- 20091117
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 114
IP  - 18
DP  - 2009 Oct 29
TI  - The human alloreactive CD4+ T-cell repertoire is biased to a Th17 response and 
      the frequency is inversely related to the number of HLA class II mismatches.
PG  - 3947-55
LID - 10.1182/blood-2009-03-211896 [doi]
AB  - Estimates of precursor frequency and assessment of functional characteristics of 
      alloreactive CD4+ T cells are all biased by the need for long-term culture. In 
      this study, direct visualization of human alloreactive CD4+ T cells on the 
      single-cell level was achieved using cell surface expression of CD154 as a tool 
      for identification. The average frequency of alloreactive CD154+CD4+ T cells 
      among peripheral blood CD4+ T cells was 0.1%, with half of the cells displaying a 
      naive phenotype. The proliferation capacity and expression of cytokines after 
      allogeneic stimulation resided in these CD154+CD4+ T cells. The repertoire of 
      alloreactive CD4+ T cells was biased to a Th17 response, and on average 24% of 
      alloreactive CD154+CD4+ memory T cells produced interleukin-17 (IL-17) after 
      polyclonal stimulation. Unexpectedly, mixed cell cultures from human leukocyte 
      antigen (HLA)-identical donors also generated alloreactive CD154+CD4+ T cells and 
      yielded the highest frequency compared with HLA-nonidentical combinations. 
      Therefore, reactivity to minor histocompatibility antigens between HLA-identical 
      subjects appears to be relatively common. Alloreactive HLA-identical T cells did 
      not proliferate or express cytokines, but were driven to proliferation in the 
      presence of exogenous IL-2.
FAU - Litjens, Nicolle H R
AU  - Litjens NH
AD  - Department of Internal Medicine, Division of Nephrology, Erasmus Medical Center, 
      Rotterdam, The Netherlands.
FAU - van de Wetering, Jacqueline
AU  - van de Wetering J
FAU - van Besouw, Nicole M
AU  - van Besouw NM
FAU - Betjes, Michiel G H
AU  - Betjes MG
LA  - eng
PT  - Journal Article
DEP - 20090827
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (IL2 protein, human)
RN  - 0 (Interleukin-2)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - *Blood Donors
MH  - CD40 Ligand
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Histocompatibility/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - *Immunologic Memory
MH  - Interleukin-2/pharmacology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Time Factors
EDAT- 2009/08/29 09:00
MHDA- 2009/11/18 06:00
CRDT- 2009/08/29 09:00
PHST- 2009/08/29 09:00 [entrez]
PHST- 2009/08/29 09:00 [pubmed]
PHST- 2009/11/18 06:00 [medline]
AID - S0006-4971(20)39032-7 [pii]
AID - 10.1182/blood-2009-03-211896 [doi]
PST - ppublish
SO  - Blood. 2009 Oct 29;114(18):3947-55. doi: 10.1182/blood-2009-03-211896. Epub 2009 
      Aug 27.