PMID- 19714581
OWN - NLM
STAT- MEDLINE
DCOM- 20091019
LR  - 20151119
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 60
IP  - 9
DP  - 2009 Sep
TI  - Urinary CD4+ effector memory T cells reflect renal disease activity in 
      antineutrophil cytoplasmic antibody-associated vasculitis.
PG  - 2830-8
LID - 10.1002/art.24747 [doi]
AB  - OBJECTIVE: Numbers of circulating CD4+ effector memory T cells are proportionally 
      increased in patients with proteinase 3 antineutrophil cytoplasmic 
      antibody-associated vasculitis (AAV) whose disease is in remission and are 
      decreased during active disease, which presumably reflects their migration toward 
      sites of inflammation. Since renal infiltrating cells may appear in urine, we 
      investigated the presence of CD4+ effector memory T cells in urinary sediment as 
      a reflection of renal disease activity in AAV. METHODS: CD4+ effector memory 
      (CD45RO+CCR7-CD3+CD4+) T cells were quantitated in the urine and peripheral blood 
      of patients with AAV with renal involvement (n = 33), patients with AAV without 
      renal involvement (n = 18), patients with AAV whose disease was in remission (n = 
      29), and patients with active disease (n = 22), using 4-color flow cytometric 
      analysis. Numbers and percentages of urine CD4+ effector memory T cells in 12 
      patients with AAV with active renal disease were obtained over several weeks of 
      followup during remission induction. RESULTS: A notable increase in urine CD4+ 
      effector memory T cell numbers was observed in patients with active renal AAV 
      compared with patients whose disease was in remission and patients with active 
      disease without renal involvement. The increase in these cells in the urine of 
      patients with active renal AAV was accompanied by a reciprocal decrease in these 
      cells in peripheral blood. Results from followup analysis showed a clear 
      reduction in urine CD4+ effector memory T cells following treatment. Moreover, a 
      negative correlation was observed between percentages of circulating and urine 
      CD4+ effector memory T cells, consistent with their migration toward sites of 
      inflammation. CONCLUSION: Our findings indicate that the presence of CD4+ 
      effector memory T cells in urine reflects renal involvement in AAV. Flow 
      cytometric analysis of these cells in urine may contribute to assessing renal 
      disease activity in patients with AAV.
FAU - Abdulahad, Wayel H
AU  - Abdulahad WH
AD  - Department of Rheumatology and Clinical Immunology, University Medical Center 
      Groningen, Hanzeplein 1, Groningen, The Netherlands. w.abdulahad@int.umcg,nl
FAU - Kallenberg, Cees G M
AU  - Kallenberg CG
FAU - Limburg, Pieter C
AU  - Limburg PC
FAU - Stegeman, Coen A
AU  - Stegeman CA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Antineutrophil Cytoplasmic/*metabolism
MH  - Biomarkers/blood/urine
MH  - CD4-Positive T-Lymphocytes/*immunology/*pathology
MH  - Cell Movement
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - Kidney Diseases/blood/pathology/*urine
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Remission Induction
MH  - Severity of Illness Index
MH  - Vasculitis/*complications/*immunology/pathology
EDAT- 2009/08/29 09:00
MHDA- 2009/10/20 06:00
CRDT- 2009/08/29 09:00
PHST- 2009/08/29 09:00 [entrez]
PHST- 2009/08/29 09:00 [pubmed]
PHST- 2009/10/20 06:00 [medline]
AID - 10.1002/art.24747 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2009 Sep;60(9):2830-8. doi: 10.1002/art.24747.