PMID- 19714869
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20151119
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 8
IP  - 4
DP  - 2009 Apr
TI  - Selective expression of Syntaxin-7 protein in benign melanocytes and malignant 
      melanoma.
PG  - 1639-46
LID - 10.1021/pr800745e [doi]
AB  - To search for proteins expressed in human melanocytes and melanoma, we employed 
      an antibody-based proteomics strategy to screen for protein expression in tissue 
      microarrays containing normal tissues, cancer tissues and cell lines. Syntaxin-7 
      (STX7) was identified as a novel protein, not previously characterized in cells 
      of melanocytic lineage, displaying a cell type-specific protein expression 
      pattern. In tumor tissues, STX7 was expressed in malignant melanoma and lymphoma. 
      The protein was further characterized regarding subcellular localization, 
      specificity, tissue distribution pattern and potential as a diagnostic and 
      prognostic marker using cell lines and tissue microarrays containing normal skin, 
      melanocytic nevi and primary and metastatic melanoma. STX7 was expressed in 
      normal melanocytes, various benign melanocytic nevi, atypical nevi and malignant 
      melanoma. Analysis in two independent melanoma cohorts demonstrated STX7 
      expression in nearly all investigated tumors, although at varying levels (> 90% 
      positive tumors). The expression level of STX7 protein was inversely correlated 
      to tumor stage, suggesting that decreased expression of STX7 is associated with 
      more aggressive tumors. In conclusion, we present protein profiling data for a 
      novel protein showing high sensitivity and specificity for cells of the 
      melanocytic lineage. The presented antibody-based proteomics approach can be used 
      as an effective strategy to identify novel tumor markers and evaluate their 
      potential clinical relevance.
FAU - Stromberg, Sara
AU  - Stromberg S
AD  - Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 
      SE-75185 Uppsala, Sweden.
FAU - Agnarsdottir, Margret
AU  - Agnarsdottir M
FAU - Magnusson, Kristina
AU  - Magnusson K
FAU - Rexhepaj, Elton
AU  - Rexhepaj E
FAU - Bolander, Asa
AU  - Bolander A
FAU - Lundberg, Emma
AU  - Lundberg E
FAU - Asplund, Anna
AU  - Asplund A
FAU - Ryan, Denise
AU  - Ryan D
FAU - Rafferty, Mairin
AU  - Rafferty M
FAU - Gallagher, William M
AU  - Gallagher WM
FAU - Uhlen, Mathias
AU  - Uhlen M
FAU - Bergqvist, Michael
AU  - Bergqvist M
FAU - Ponten, Fredrik
AU  - Ponten F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Qa-SNARE Proteins)
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - Cell Line, Tumor
MH  - Humans
MH  - Melanocytes/*metabolism
MH  - Melanoma/*metabolism
MH  - Qa-SNARE Proteins/*metabolism
MH  - Skin/*metabolism
MH  - Skin Neoplasms/*metabolism
EDAT- 2009/08/29 09:00
MHDA- 2009/10/02 06:00
CRDT- 2009/08/29 09:00
PHST- 2009/08/29 09:00 [entrez]
PHST- 2009/08/29 09:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - 10.1021/pr800745e [pii]
AID - 10.1021/pr800745e [doi]
PST - ppublish
SO  - J Proteome Res. 2009 Apr;8(4):1639-46. doi: 10.1021/pr800745e.