PMID- 19717682
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20090831
IS  - 1477-9145 (Electronic)
IS  - 0022-0949 (Linking)
VI  - 212
IP  - 18
DP  - 2009 Sep 15
TI  - The response of non-traditional natriuretic peptide production sites to salt and 
      water manipulations in the rainbow trout.
PG  - 2991-7
LID - 10.1242/jeb.031666 [doi]
AB  - Natriuretic peptides (NPs) and their receptors (NPRs) comprise an evolutionarily 
      conserved signaling system with profound physiological effects on vertebrate 
      renal and cardiovascular systems. Some NPs (ANP, BNP and VNP) are primarily of 
      cardiac origin whereas CNP is common in the brain. In mammals, non-traditional 
      sites of NPs synthesis, BNP in brain and CNP in atrium, appear to have 
      complementary actions. In the present study, trout were chronically adapted to 
      freshwater (FW) (a volume-loading, salt-depleting environment), saltwater (SW) (a 
      volume-depleting, salt-loading environment), FW and fed a high-salt diet (FW-HSD) 
      (a volume- and salt-loading regime) or acutely volume depleted or expanded by 
      hemorrhage or infusion with dialyzed plasma to perturb volume homeostasis. The 
      responses of brain and atrial BNP and CNP mRNA, pro-peptide, NPR-A and NPR-B were 
      evaluated using quantitative PCR and western analysis. Brain pro-BNP and NPR-A 
      was increased in FW-HSD trout and decreased in SW trout. Brain pro-CNP was 
      largely unaffected whereas NPR-B mRNA was increased in FW-HSD trout. Atrial CNP, 
      although produced at lower levels than other cardiac NPs, was markedly elevated 
      in chronically (FW-HSD) and acutely volume expanded trout (dialyzed-plasma 
      infusion) whereas decreased in hemorrhaged trout. These findings indicate that 
      non-traditional NP synthesis sites in the trout probably complement the broad 
      hypovolemic and hypotensive actions of traditional (cardiac) NP synthesis sites 
      in response to volume expansion but not to plasma osmolarity. This supports the 
      hypothesis that the piscine and mammalian NP systems are fundamentally similar 
      and appear to protect the heart from volume overload.
FAU - Johnson, Keven R
AU  - Johnson KR
AD  - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 
      46556, USA.
FAU - Olson, Kenneth R
AU  - Olson KR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - 0 (Fish Proteins)
RN  - 0 (Natriuretic Peptides)
RN  - 0 (Protein Isoforms)
RN  - 0 (Sodium Chloride, Dietary)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Fish Proteins/biosynthesis/genetics
MH  - *Fresh Water
MH  - Natriuretic Peptides/*biosynthesis/genetics
MH  - Oncorhynchus mykiss/*physiology
MH  - Protein Isoforms/*biosynthesis/genetics
MH  - *Seawater
MH  - Sodium Chloride, Dietary
MH  - Tissue Distribution
MH  - Water-Electrolyte Balance
EDAT- 2009/09/01 06:00
MHDA- 2009/12/18 06:00
CRDT- 2009/09/01 09:00
PHST- 2009/09/01 09:00 [entrez]
PHST- 2009/09/01 06:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
AID - 212/18/2991 [pii]
AID - 10.1242/jeb.031666 [doi]
PST - ppublish
SO  - J Exp Biol. 2009 Sep 15;212(18):2991-7. doi: 10.1242/jeb.031666.