PMID- 19718776
OWN - NLM
STAT- MEDLINE
DCOM- 20091027
LR  - 20191210
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 23
IP  - 19
DP  - 2009 Oct
TI  - Study of human neutrophil peptides in saliva by matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry.
PG  - 3220-6
LID - 10.1002/rcm.4241 [doi]
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry is used to rapidly characterize the human neutrophil peptides - HNP 
      1, 2, and 3 - in saliva. The saliva excreted from the parotid and 
      sublingual/submandibular glands of 70 individuals were collected and examined 
      using MALDI-TOF. The MALDI approach requires no sample pretreatment other than 
      mixing the saliva-absorbing material with the matrix and drying under ambient 
      conditions. Tissue paper was the best material for collecting the saliva samples 
      because of its strong texture and high absorbance, and sinapinic acid was the 
      best MALDI matrix for the analysis of the HNPs. HNPs were detected in almost all 
      the samples collected from the parotid glands, with no obvious differences among 
      age or gender. In contrast, the distribution of the HNPs in the samples collected 
      from the sublingual/submandibular glands was age-dependent: no HNPs were detected 
      for those collected from individuals younger than 30, but the HNPs were present 
      in all of the samples collected from those older than 60 years. The increased 
      probability of detecting saliva HNPs with age suggests that HNPs may function as 
      a biomarker for aging.
CI  - Copyright (c) 2009 John Wiley & Sons, Ltd.
FAU - Yang, Ming-Hui
AU  - Yang MH
AD  - Department of Chemistry, National Sun Yat-Sen University, Taiwan.
FAU - Lo, Li-Hua
AU  - Lo LH
FAU - Chen, Yi-Hsuan
AU  - Chen YH
FAU - Shiea, Jentaie
AU  - Shiea J
FAU - Wu, Pei-Chang
AU  - Wu PC
FAU - Tyan, Yu-Chang
AU  - Tyan YC
FAU - Jong, Yuh-Jyh
AU  - Jong YJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (alpha-Defensins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Saliva/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Young Adult
MH  - alpha-Defensins/*chemistry
EDAT- 2009/09/01 06:00
MHDA- 2009/10/29 06:00
CRDT- 2009/09/01 09:00
PHST- 2009/09/01 09:00 [entrez]
PHST- 2009/09/01 06:00 [pubmed]
PHST- 2009/10/29 06:00 [medline]
AID - 10.1002/rcm.4241 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2009 Oct;23(19):3220-6. doi: 10.1002/rcm.4241.