PMID- 19719646
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20090901
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 44
IP  - 1
DP  - 2003 May 1
TI  - Factors influencing the detection of bacterial cells using fluorescence in situ 
      hybridization (FISH): A quantitative review of published reports.
PG  - 3-15
LID - 10.1016/S0168-6496(02)00461-0 [doi]
AB  - Abstract Fluorescence in situ hybridization (FISH) is widely used to describe 
      bacterial community composition and, to a lesser extent, to describe the 
      physiological state of cells. One of the limitations of the technique is that the 
      effectiveness of the detection of target cells appears to vary widely. Here, we 
      present a quantitative review of published reports on the percentage of cells 
      detected using the common EUB338 probe (%Eub) in aquatic ecosystems. The %Eub 
      varies from 1 to 100% in the different published reports, with an average of 56%. 
      There is a methodological component in this variation, with a significant effect 
      of the fluorochrome type and the stringency conditions of the reaction. But there 
      is also a strong environmental component, and the type of ecosystem and dominant 
      phylogenetic group significantly influence %Eub. We argue that the optimization 
      of the FISH protocol to describe the phylogenetic composition of bacterial 
      assemblages will probably lead to techniques that are not effective to describe 
      the physiological state of cells.
FAU - Bouvier, Thierry
AU  - Bouvier T
AD  - Dept. des Sciences Biologiques, Universite du Quebec a Montreal, CP 8888, Succ. 
      Centre Ville, Montreal, QC, Canada H3C 3P8.
FAU - Del Giorgio, Paul A
AU  - Del Giorgio PA
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
EDAT- 2003/05/01 00:00
MHDA- 2003/05/01 00:01
CRDT- 2009/09/02 09:00
PHST- 2009/09/02 09:00 [entrez]
PHST- 2003/05/01 00:00 [pubmed]
PHST- 2003/05/01 00:01 [medline]
AID - FEM3 [pii]
AID - 10.1016/S0168-6496(02)00461-0 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2003 May 1;44(1):3-15. doi: 10.1016/S0168-6496(02)00461-0.