PMID- 19719673
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20091211
LR  - 20090901
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 43
IP  - 2
DP  - 2003 Mar 1
TI  - Gastrointestinal microbial community shifts observed following oral 
      administration of a Lactobacillus fermentum strain to mice.
PG  - 133-40
LID - 10.1111/j.1574-6941.2003.tb01052.x [doi]
AB  - The indigenous gastrointestinal microbiota acts as an integral defense against 
      the colonisation of orally introduced microbes. Whilst this can be important in 
      host protection, some introduced species, including lactobacilli, can have a 
      positive impact on existing microbial communities. The interaction of a candidate 
      probiotic strain of Lactobacillus fermentum within the gastrointestinal tract was 
      monitored in a mouse model and its effect on the indigenous microbiota observed. 
      L. fermentum KLD was administered via oro-gastric doses to mice with both a 
      specific pathogen-free (SPF) and an ampicillin-depleted gut microbiota, 
      containing no detectable lactobacilli. Its persistence was monitored by detection 
      in faecal homogenates using culturing methods and polymerase chain reaction with 
      L. fermentum specific primers. Microbial population shifts were observed using 
      denaturing gradient gel electrophoresis (DGGE). L. fermentum KLD was detected 
      within the gastrointestinal tract of SPF mice for up to 36 h, and for greater 
      than 11 days in the ampicillin-treated mice. The administration resulted in 
      substantial changes within the host Lactobacillus levels, determined by DGGE of 
      16S rDNA from faecal samples. Denaturing gradient profiles, from faecal samples 
      collected at a range of pre- and post-dose intervals of groups of 10 SPF mice, 
      indicated that several other constituents of the gastrointestinal community also 
      fluctuated following dosing. These included Bifidobacterium and Eubacterium, 
      which increased following KLD administration. The indigenous microbiota affected 
      the persistence of L. fermentum KLD and in SPF mice the administration of this 
      strain induced significant shifts in the indigenous microbial community.
FAU - Plant, Laura
AU  - Plant L
AD  - School of Microbiology and Immunology, The University of New South Wales, Sydney, 
      NSW 2052, Australia.
FAU - Lam, Chi
AU  - Lam C
FAU - Conway, Patricia L
AU  - Conway PL
FAU - O'Riordan, Katie
AU  - O'Riordan K
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
EDAT- 2003/03/01 00:00
MHDA- 2003/03/01 00:01
CRDT- 2009/09/02 09:00
PHST- 2009/09/02 09:00 [entrez]
PHST- 2003/03/01 00:00 [pubmed]
PHST- 2003/03/01 00:01 [medline]
AID - FEM133 [pii]
AID - 10.1111/j.1574-6941.2003.tb01052.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2003 Mar 1;43(2):133-40. doi: 
      10.1111/j.1574-6941.2003.tb01052.x.