PMID- 19719701 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20121002 LR - 20090901 IS - 1574-6941 (Electronic) IS - 0168-6496 (Linking) VI - 43 IP - 1 DP - 2003 Feb 1 TI - Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis. PG - 111-9 LID - 10.1111/j.1574-6941.2003.tb01050.x [doi] AB - Abstract Physiologic activity and community structure of planktonic and biofilm microbial communities in an urban river were analyzed using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) staining, fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) analysis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Respiring bacteria estimated by CTC reduction were higher in biofilms (20%) than in stream water samples (12%). FISH analysis revealed that bacterial populations in both stream water and biofilms were dominated by beta-Proteobacteria and Cytophaga-Flavobacterium cluster. Microbial community changes determined by multidimensional scaling analysis from DGGE patterns showed that microbial community structures in biofilms matured within 3-7 days of their formation and did not change further, while those in stream water changed continuously. FAU - Araya, Ruben AU - Araya R AD - Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan. FAU - Tani, Katsuji AU - Tani K FAU - Takagi, Tatsuya AU - Takagi T FAU - Yamaguchi, Nobuyasu AU - Yamaguchi N FAU - Nasu, Masao AU - Nasu M LA - eng PT - Journal Article PL - England TA - FEMS Microbiol Ecol JT - FEMS microbiology ecology JID - 8901229 EDAT- 2003/02/01 00:00 MHDA- 2003/02/01 00:01 CRDT- 2009/09/02 09:00 PHST- 2009/09/02 09:00 [entrez] PHST- 2003/02/01 00:00 [pubmed] PHST- 2003/02/01 00:01 [medline] AID - FEM111 [pii] AID - 10.1111/j.1574-6941.2003.tb01050.x [doi] PST - ppublish SO - FEMS Microbiol Ecol. 2003 Feb 1;43(1):111-9. doi: 10.1111/j.1574-6941.2003.tb01050.x.