PMID- 19720044
OWN - NLM
STAT- MEDLINE
DCOM- 20091030
LR  - 20131121
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 490
IP  - 2
DP  - 2009 Oct 15
TI  - Apoptotic inducers activate the release of D-aspartate through a hypotonic 
      stimulus-triggered mechanism in PC12 cells.
PG  - 118-28
LID - 10.1016/j.abb.2009.08.017 [doi]
AB  - We have characterized release of D-aspartate (D-Asp), a regulator of hormone 
      synthesis and secretion, via a volume-sensitive organic anion channel (VSOC) in 
      PC12 cells by studying its response to apoptotic stimuli. PC12 cells have been 
      demonstrated to endogenously synthesize D-Asp. Apoptotic inducers, including 
      staurosporin (STS), tumor necrosis factor (TNF)-alpha, H(2)O(2), and C2-ceramide, 
      activate the release of D-Asp through a hypotonic stimulus-triggered mechanism. 
      Putative blockers of the anion channel, 5-nitro-2-(3-phenylpropylamino)benzoic 
      acid (NPPB) and 4,4'-diisothiocyanostilbene-2,2'-sulphonic acid (DIDS), 
      significantly inhibited stress-induced D-Asp release under hypotonic conditions 
      following the application of apoptotic inducers. Hypotonic conditions are 
      essential for activation by apoptotic inducers. Phorbol 12-mirystate 13-acetate 
      and the Ca(2+) ionophore A23187 increased D-Asp efflux via the VSOC, implying the 
      involvement of intracellular Ca(2+) in the activation of the D-Asp efflux. 
      However, hypotonic stress and STS had no effect on the concentration of 
      intracellular Ca(2+) in PC12 cells. Furthermore, an unknown EGTA-sensitive 
      factor(s), other than Ca(2+), and peroxynitrite may play pivotal roles in 
      STS-enhanced D-Asp release.
FAU - Furuchi, Takemitsu
AU  - Furuchi T
AD  - Laboratory of Biomolecular Science, Department of Pharmaceutical Life Sciences, 
      Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
FAU - Suzuki, Toshiyuki
AU  - Suzuki T
FAU - Sekine, Masae
AU  - Sekine M
FAU - Katane, Masumi
AU  - Katane M
FAU - Homma, Hiroshi
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090829
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Hypotonic Solutions)
RN  - 0 (Ion Channels)
RN  - 0 (Ionophores)
RN  - 0 (N-acetylsphingosine)
RN  - 0 (Nitrobenzoates)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 268B43MJ25 (Uric Acid)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 3A35O9G3YZ (5-nitro-2-(3-phenylpropylamino)benzoic acid)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - H88EPA0A3N (Staurosporine)
RN  - NGZ37HRE42 (Sphingosine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/*physiology
MH  - Aspartic Acid/chemistry/*metabolism
MH  - Calcimycin/pharmacology
MH  - Hydrogen Peroxide/pharmacology
MH  - Hypotonic Solutions
MH  - Ion Channels/drug effects/metabolism
MH  - Ionophores/pharmacology
MH  - Nitrobenzoates/pharmacology
MH  - PC12 Cells
MH  - Rats
MH  - Sphingosine/analogs & derivatives/pharmacology
MH  - Staurosporine/pharmacology
MH  - Stereoisomerism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Uric Acid/pharmacology
EDAT- 2009/09/02 06:00
MHDA- 2009/10/31 06:00
CRDT- 2009/09/02 09:00
PHST- 2009/06/24 00:00 [received]
PHST- 2009/08/25 00:00 [revised]
PHST- 2009/08/27 00:00 [accepted]
PHST- 2009/09/02 09:00 [entrez]
PHST- 2009/09/02 06:00 [pubmed]
PHST- 2009/10/31 06:00 [medline]
AID - S0003-9861(09)00278-1 [pii]
AID - 10.1016/j.abb.2009.08.017 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2009 Oct 15;490(2):118-28. doi: 10.1016/j.abb.2009.08.017. 
      Epub 2009 Aug 29.